计算溶液所需的质量、体积或浓度。
有货
为什么选择此级别
BioReagent,天然 级 提供 ≥55%(GE) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 2 篇同行评审文献引用。
概述
Triticum vulgaris lectin or Wheat germ agglutinin (WGA) is isolated from Triticum vulgaris (Wheat germ) and purified by affinity chromatography. The lectin has two subunits and a molecular weight of 36 kDa. WGA selectively binds to N-Acetyl glucosamine (GlcNAc) and to N-acetylneuraminic acid (sialic acid) residues of glycoproteins and glycolipids.
The WGA lectin agglutinates erythrocytes and most types of malignant cells. It agglutinates rabbit erythrocytes at < 0.1 µg/ml following trypsin treatment of the cells. Adding 300 mM N-Acetyle-D-Glucosamine gives an inhibition with a titer that is at least 8-fold lower than the control.
Wheat germ agglutinin inhibits the C5a receptor interaction, which has implications in studies of receptor micro-heterogeneity and ligand binding sites.
WGA together with Con A is the lectin most widely used as an analytical and preparative agent when studying glycoproteins and cell surface proteins. The immobilized lectin can be used for affinity chromatography of cells and sub-cellular particles.
Aladdin’s WGA is supplied as a white to pale yellow lyophilized powder from a buffer containing 10 mM CH₃COONH₄. No preservatives are added. The purity of WGA is determined by SDS- electrophoresis which generates three bands, corresponding to the three isolectins of WGA. Protein content is verified in spectrophotmetry.
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Benefits
• Ultrapure quality
• Sugar specificity: N-acetylglucosamine and N-acetylneuraminic acid residues
• Agglutinates erythrocytes and most types of malignant cells
• Agglutinates rabbit erythrocytes at ≤0.1 µg/ml after treating the cells with trypsin
• Not blood group specific
Application:
• Studies of glycoproteins and glycolipids
• Purification of membrane proteins
• Affinity chromatography of cells and sub-cellular particles
Directions for use
The lectin may be reconstituted with 2 ml of deionized water before use, spin the vial gently until full dissolution. The solution may be reconstituted in this buffer to desired working concentration. Storage at pH 8.6–8.8 causes precipitation.
Tips and hints
Avoid repeated freezing and thawing.
Shipping and storage
The product is shipped at -20°C however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than three years from
production date when stored below -20°C. After reconstitution with deionized water, the solution may be stored frozen in working aliquots for up to 12 months.
规格
产品名称
麦胚凝集素 (WGA)
别名
来自普通小麦的凝集素 | 小麦胚芽凝集素
规格或纯度
BioReagent,天然,≥55%(GE),Protein content ≥85%
生化机理
Lectin is known to be useful in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling. This is because of its specific interaction with oligosaccharides, glycoproteins and glycolipids. In plants and fungi, lectin defends against pathogens/feeders. Lectin participates in host recognition and tissue adhesion, thereby aids in the pathogenesis of microorganism.
生物活性
Agglutinates erythrocytes and most types of malignant cells.
种属
普通小麦(小麦胚芽)(Triticum vulgaris (Wheat germ))
来源
天然
预测分子量
36 kDa
CAS编号和信息
96508-08-6
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 10 mM CH₃COONH₄.
浓度
Protein content ≥85%
复溶
Reconstitute in 0.9% NaCl. PBS or TBS are also suitable alternatives for reconstitution.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
长期储存-20℃（3年）；复溶后建议分装，避免反复冻融。
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0838269 |
分析证书 | L755067 |
| ZJ26F0333358 |
分析证书 | L755067 |
| ZJ26F0333359 |
分析证书 | L755067 |
| ZJ26F0333360 |
分析证书 | L755067 |
技术文档和文章
此产品的引用文献
溶液计算器
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