计算溶液所需的质量、体积或浓度。
丙烯酸酯化泊洛沙姆（F127DA）
有货
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
F127DA是由泊洛沙姆407（F127）两端羟基经丙烯酰基修饰得到的、具有光敏特性的高分子材料，可与蓝光或紫外光引发剂配合使用，在蓝光或紫外光辐照下交联固化。该高分子具有优异的生物相容性、温敏性和可自组装的特性，且固化简单，形成的水凝胶力学性能良好，可以承载较大应力，可用在药物载体、高强度水凝胶制备、组织工程、伤口敷料、生物3D打印、微流控芯片制备等领域。
产品规格
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使用建议
1、F127DA可与邻硝基苄醇化高分子，如HANB联合使用。二者由光引发剂光照引发聚合并交联，仅需数秒即可获得高强度水凝胶材料。
2、F127DA可与甲基丙烯基修饰的高分子，如CMCMA、HAMA、CSMA、ChMA以及丙烯基修饰的高分子，如PEGDA联合使用，由光引发剂光照引发聚合，构建不同理化性能的水凝胶材料。
3、F127DA单独使用，由光引发剂光照引发聚合并交联。
配制方法
1、配制光引发剂标准液：取一定质量的苯基（2,4,6-三甲基苯甲酰基）亚膦酸钠（NAP）配制成浓度为0.1wt% - 0.25wt%范围内的溶液，避光保存；
2、取所需质量的F127DA放入离心管，并取引发剂标准溶液加入到上述离心管中，涡旋使F127DA充分浸润；
3、当溶液浓度≤15wt%时，可将样品置于室温下搅拌或涡旋溶解；当溶液浓度大于15wt%时，可将样品于2-8℃静置溶解，期间用漩涡仪振荡数次，至样品完全溶解。
注意事项
浓度大于15wt%的F127DA溶液，在室温静置会出现凝胶化现象，浓度越高，越易凝胶化。将温度降低到2~8℃后会重新恢复溶液状态。
产品应用
药物载体、高强度水凝胶制备、组织工程、伤口敷料、生物3D打印、微流控芯片制备等。
灭菌方式
1、过滤灭菌（建议）：使用0.22μm无菌针头过滤器过滤溶液灭菌。
2、巴氏灭菌：将溶液加热到80℃，保持30min；再迅速转移至冰水混合物中冷却至常温。共循环上述操作三次。
3、湿热灭菌：将溶液于高温高压灭菌锅内，121℃下灭菌8min，手动放气后，将溶液迅速转移至冰水混合物中冷却至常温。
4、无菌溶液可以2-8℃避光暂存，建议7天内使用；非无菌溶液可以2-8℃暂存，建议48小时内使用。
F127DA产品特点
1、可自组装：
高分子链的两端由亲水性的PEO组成，而链的中段则由相对疏水的PPO构成。这种独特的结构赋予了该材料在水中自组装成纳米胶束的能力，其中疏水性的PPO部分会聚集在胶束的内部，形成一个局部的疏水环境，而亲水性的PEO部分则因其极性特性而伸展到胶束的外部。基于这些性质，该材料可用于增加疏水性药物的溶解度。
2、温敏性：可注射
F127DA在低温下呈现液态，而在接近体温范围内（约20°C至40°C）转变为凝胶态。因此可用于注射、实现对药物的缓慢释放和控制释放。此外，还可用于3D生物打印墨水制备。
3、光敏性：原位构建高弹性、低溶胀水凝胶材料
基于自组装的特性，F127DA可形成纳米微颗粒分散在水溶液中，同时裸露在纳米颗粒外部的双键官能团使其可作为交联剂参与反应，从而构建高强度复合水凝胶。且疏水链段的存在使得该水凝胶材料在37℃时几乎不发生溶胀。
F127DA典型应用
1、疏水药物负载秒级固化、高强、高粘附光交联水凝胶实现心脏穿刺口的止血
2、喉软骨缺损修复：构建低溶胀、高抗压水凝胶实现喉软骨缺损的修复
3、打印多孔假体：多孔假体与脂肪细胞混合植入实现乳房重建
F127DA产品优势
1、批次稳定：采用标准化的生产工艺，保证不同批次的F127DA在成分、理化特性和生物学方面具有高度的重复性和可控性。
2、超高纯度：采用独特的生产工艺，有效地去除甲基丙烯酸小分子和工艺杂质，实现超低杂质水平，保证F127DA在生物医学应用的安全性。
3、可靠的安全性：每批产品均经过严格的标准化的检验后放行，保证F127DA在生物医学应用的安全性。
4、高稳定性：参照《无源植入性医疗器械稳定性研究指导原则》，经过实时稳定性和加速稳定性试验证明：F127DA产品在标示贮存条件下质量稳定。
F127DA is a photosensitive polymer synthesized by acrylation modification of the terminal hydroxyl groups of Poloxamer 407 (F127). It can be used together with blue light or UV photoinitiators and crosslinks to form a solid network upon irradiation with blue light or ultraviolet light. This polymer features excellent biocompatibility, thermoresponsiveness and self-assembly capability. It cures easily and yields hydrogels with favorable mechanical properties capable of bearing high mechanical stress. It finds applications in drug delivery carriers, high-strength hydrogel fabrication, tissue engineering, wound dressings, biological 3D bioprinting, microfluidic chip manufacturing and other related fields.
Product Specifications
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Recommended Operation
1. F127DA can be used in combination with o-nitrobenzyl-functionalized polymers such as HANB. The two components undergo photopolymerization and crosslinking triggered by photoinitiators under light irradiation, yielding high-strength hydrogel materials within only a few seconds.
2. F127DA can be blended with methacrylated polymers (CMCMA, HAMA, CSMA, ChMA) and acrylated polymers (e.g. PEGDA). Photopolymerization initiated by photoinitiators under light exposure enables fabrication of hydrogels with diversified physicochemical properties.
3. F127DA can be used independently to achieve photopolymerization and crosslinking with the aid of photoinitiators under light irradiation.
Preparation Protocol
1. Preparation of standard photoinitiator solution: Weigh a certain amount of sodium phenyl (2,4,6-trimethylbenzoyl) phosphinate (NAP) and dissolve it to prepare a solution with a mass fraction of 0.1 wt%–0.25 wt%. Store the solution away from light.
2. Transfer the required mass of F127DA into a centrifuge tube, add the standard initiator solution into the tube, and vortex thoroughly to fully wet F127DA.
3. For solutions with concentration ≤15 wt%, dissolve the sample by stirring or vortexing at room temperature. For concentrations above 15 wt%, place the sample at 2–8 °C for static dissolution, and vortex several times intermittently until complete dissolution.
Precautions
F127DA solutions with concentrations higher than 15 wt% tend to gel when kept at room temperature; higher concentrations lead to more pronounced gelation. The system will revert to a liquid state after cooling to 2–8 °C.
Product Applications
Drug delivery carriers, fabrication of high-strength hydrogels, tissue engineering, wound dressings, biological 3D bioprinting, microfluidic chip manufacturing, etc.
Sterilization Methods
1. Sterile filtration (Recommended): Sterilize the solution by filtration with a 0.22 μm sterile syringe filter.
2. Pasteurization: Heat the solution to 80 °C and hold for 30 min, then quickly transfer it into an ice-water mixture to cool to room temperature. Repeat the above process for three cycles in total.
3. Moist heat sterilization: Place the solution in an autoclave and sterilize at 121 °C for 8 min. After manual pressure release, immediately transfer the solution to an ice-water mixture and cool to room temperature.
4. Sterile solutions can be temporarily stored at 2–8 °C protected from light and are recommended for use within 7 days; non-sterile solutions can be temporarily stored at 2–8 °C and are recommended for use within 48 hours.
Product Features of F127DA
1. Self-assembly Property
The polymer chain is composed of hydrophilic PEO segments at both ends and relatively hydrophobic PPO segments in the middle. This unique structure enables the material to self-assemble into nanomicelles in water. The hydrophobic PPO segments aggregate inside the micelles to form a localized hydrophobic microenvironment, while the polar hydrophilic PEO segments extend outward to the micelle surface. Benefiting from such properties, this material can improve the solubility of hydrophobic drugs.
2. Thermoresponsiveness & Injectability
F127DA exists as a liquid at low temperatures and undergoes a liquid-to-gel transition near body temperature (approximately 20 °C to 40 °C). Therefore, it is injectable to achieve sustained and controlled drug release. Besides, it can be applied to formulate bioinks for 3D bioprinting.
3. Photosensitivity: In-situ fabrication of highly elastic, low-swelling hydrogels
Relying on its self-assembly capability, F127DA can form dispersed nanoparticles in aqueous solution. The double bond functional groups exposed on the nanoparticle surface allow F127DA to act as a crosslinker for fabricating high-strength composite hydrogels. Moreover, the presence of hydrophobic segments renders the hydrogel nearly non-swelling at 37 °C.
Typical Applications of F127DA
1. Loading of hydrophobic drugs; instant photo-crosslinkable hydrogels with high mechanical strength and strong adhesion for hemostasis at cardiac puncture wounds
2. Laryngeal cartilage defect repair: Fabrication of low-swelling and high-compressive-strength hydrogels to repair laryngeal cartilage defects.
3. Porous scaffold bioprinting: Implantation of porous scaffolds mixed with adipocytes for breast reconstruction.
Product Advantages of F127DA
1. Consistent batch performance: Standardized manufacturing processes ensure high repeatability and controllability of the chemical composition, physicochemical properties and biological performance of F127DA across different batches.
2. Ultra-high purity: Proprietary production processes efficiently remove small-molecule methacrylic acid and process impurities to achieve an ultra-low impurity profile, safeguarding the safety of F127DA for biomedical applications.
3. Proven biosafety: Every batch undergoes rigorous standardized testing prior to release to guarantee safety for biomedical use.
4. Excellent stability: Real-time and accelerated stability tests are conducted in accordance with the Guidelines for Stability Studies of Passive Implantable Medical Devices, verifying that F127DA maintains stable quality under the labeled storage conditions.
规格
别名
普兰尼克F127 | 泊洛沙姆407 | 聚环氧乙烷-聚环氧丙烷-聚环氧乙烷（PEO-PPO-PEO）两亲性三嵌段聚合物
英文别名
Pluronic F127 | Poloxamer407 | F127DA
规格或纯度
标记率＞95%; 丙烯酸残留≤100ppm
稳定性与储存
室温，6个月；2-8℃，12个月；-20℃，两年；避光；避免反复冻融。
英文名称
Acrylated Poloxamer (F127DA)
储存条件
避光,-20°C储存
运输条件
超低温运输
产品线
生物产品
名称和识别符
分子类型
小分子
技术文档
高级数据
产品属性
| pH | 5.0-7.5 |
|---|
化学和物理性质
敏感性
Light sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0838443 |
分析证书 | P775160 |
| ZJ26F0838442 |
分析证书 | P775160 |
| ZJ26F0434393 |
分析证书 | P775160 |
| ZJ26F0333715 |
分析证书 | P775160 |
| ZJ26F0131077 |
分析证书 | P775160 |
| ZJ25F1230169 |
分析证书 | P775160 |
| ZJ25F0521875 |
分析证书 | P775160 |
技术文档和文章
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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