计算溶液所需的质量、体积或浓度。
Western快速转膜液(20X, 湿转)
缺货
,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
| 本产品采用全新快速转印配方,能在15-40 min内完成Western Blot湿转法转膜,产热量小,可无需冰浴。转膜液稀释后直接使用,兼容甲醇和乙醇。兼容Tris-Gly体系、Hepes体系、Bis-Tris体系等多种体系自配胶和预制胶,兼容PVDF膜和NC膜。 | |||||||||||||
| 注意事项: | |||||||||||||
| 2.PVDF 膜使用前要用无水乙醇或甲醇活化。 1.实验时如果室温较高,转印槽内可以使用冰盒降温,无需冰浴。 | |||||||||||||
| 使用说明: | |||||||||||||
1000 mL快速转膜液的配制(1×)(两种方法效果一致,请根据使用习惯选择):
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| 转膜条件 | |||||||||||||
1.转膜效率与胶浓度有关,对于各种浓度 1.0 mm PAGE胶,采用恒流 400 mA,按如下时长即可将150 kDa 以下蛋白转印完全,若分子量大于 150kDa,需延长转膜时间 5~10 min:
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| 2.对于1.5 mmPAGE胶,可将上述表中转膜时间相应延长5~10 min。 |
| Product Introduction: | |||||||||||||
This product adopts a new rapid transfer formula, which can complete Western Blot wet transfer within 15-40 minutes, with low heat generation and no need for an ice bath. Dilute the transfer solution and use it directly, compatible with methanol and ethanol. Compatible with Tris Gly system, Hepes system, Bis Tris system and other self-adhesive and prefabricated adhesives, compatible with PVDF membrane and NC membrane. | |||||||||||||
Precautions: | |||||||||||||
| 1. If the room temperature is high during the experiment, an ice box can be used to cool down the transfer tank without the need for an ice bath. 2. PVDF membrane should be activated with anhydrous ethanol or methanol before use. | |||||||||||||
Instructions for Use: | |||||||||||||
| Preparation of 1000 mL rapid membrane transfer solution (1 ×) (both methods have the same effect, please choose according to usage habits): | |||||||||||||
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Film transfer conditions: | |||||||||||||
1. The transfer efficiency is related to the gel concentration. For various concentrations of 1.0 mm PAGE gel, a constant current of 400 mA can be used for the following duration to completely transfer proteins below 150 kDa. If the molecular weight is greater than 150 kDa, the transfer time needs to be extended by 5-10 minutes:
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| 2. For 1.5 mm PAGE gel, the transfer time in the above table can be extended by 5-10 minutes accordingly. |
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | T751592 | |
| 分析证书 | T751592 | |
| 分析证书 | T751592 | |
| 分析证书 | T751592 |