Agglutinates human bloodgroup O erytrocytes at a lectin concentration down to ≤ 7 μg/ml. The agglutination is inhibited by 100 mM N-acetylgalactosamine.
凝集素浓度低至≤7 μg/mL 即可凝集人 O 型血红细胞;该凝集反应可被100 mM N‑乙酰半乳糖胺抑制
微生物限度
≤100 CFU/g
保质期
‑20 ℃条件下保存≥5 年
The binding specificity of Wisteria floribunda lectin (WFL) is not completely clear but this lectin appears to preferentially bind carbohydrate structures terminating in N-acetylgalactosamine linked α or β to the 3 or 6 position of galactose. This lectin has been used to fractionate lymphocyte populations, and although not mitogenic, elicits the production of lymphokines from murine splenocytes.
Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine
Specifications
Storage Instructions
2–8 °C
Sugar Specificity
N-Acetylgalactosamine
Usage Summary
Although many buffers can be employed for reconstituting this lectin, we recommend 10 mM HEPES buffered saline, 0.1 mM CaCl₂, pH 8.5. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Agglutinates human bloodgroup O erytrocytes at a lectin concentration down to ≤ 7 μg/ml. The agglutination is inhibited by 100 mM N-acetylgalactosamine.