计算溶液所需的质量、体积或浓度。
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产品介绍:
Western免冰浴快速转膜液(10×)是一种缓冲能力更强、蛋白转膜更彻底、无需冰浴及转膜时间更短的湿转法转膜液。本产品安全无毒,通常30 min可完成转膜过程,且稀释成1× 可重复利用2-3次。 限定稀释成1×后,每次使用1000 mL转膜液进行电转,且重复利用3次情况下,本产品可以使用15次。
注意事项:
1. 本产品仅限科研使用。 2. 为了您的安全和健康,请穿实验服并戴一次性手套操作。
应用范围:
Western 转膜液
使用方法:
1. 配制方法 1000 mL 快速转膜缓冲液(1×):100 mL Western 免冰浴快速转膜液(10×)+ 700 mL 去离子水+ 200 mL 甲醇。
2. 转膜条件
设定恒流 400 mA,<150 kDa 蛋白电转 30 min 可完全转印。
注:① PVDF 膜使用前需要使用无水甲醇浸润 30 s 以上。② 不同胶浓度不同转印蛋白大小转印时间需要摸索调整。
Product introduction:
Western ice free bath rapid film transfer solution (10 ×) It is a kind of wet transfer membrane solution with stronger buffer capacity, more thorough protein transfer membrane, no ice bath and shorter transfer membrane time. This product is safe and non-toxic. Generally, the film transfer process can be completed in 30 min and diluted into 1 × It can be reused for 2-3 times. Limiting dilution to 1 × After that, 1000 ml of film transfer liquid is used for electro rotation each time, and the product can be used for 15 times when it is reused for 3 times.
Matters needing attention:
1. this product is only for scientific research. 2. for your safety and health, please wear experimental clothes and disposable gloves.
Scope of application:
Western membrane transfer solution
Instruction:
1.Making method 1000 mL rapid transfer buffer ( 1 × ) : 100 mL Western ice-free rapid transfer solution ( 10 × ) + 700 mL deionized water + 200 mL methanol.
2.Transmembrane conditions
The constant current was set to 400 mA, and the < 150 kDa protein could be completely transferred for 30 min.
Note : ①PVDF membrane needs to be soaked with anhydrous methanol for more than 30 s before use.② The transfer time of different gel concentration and different transfer protein size needs to be adjusted.