花生凝集素 (PNA)

货号: A140087
有货
级别和纯度: BioReagent ? 生物试剂级(BioReagent)—— 经测试适用于生命科学和分子生物学。适用于需要生物相容性的细胞培养、检测和生化工作。 天然 ? 天然型(Native)—— 处于天然(非重组、非变性)形态的蛋白/生物分子。当需要天然结构和活性时使用。 Protein content ≥85%
生物活性
Agglutinates rabbit erythrocytes at < 0.1 µg/ml. Agglutination fully inhibited by 10 mM D-galatose
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规格
库存
价格
数量
1mg
A140087-1mg
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¥499.90
5mg
A140087-5mg
期货 Stock Image
¥1,399.90
25mg
A140087-25mg
期货 Stock Image
¥5,199.90
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为什么选择此级别

BioReagent,天然 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述


  Arachis hypogaea lectin or Peanut Agglutinin (PNA) is isolated from peanuts and purified by affinity chromatography. The lectin has a molecular weight of 110 kDa and consists of four identical subunits of approximately 27 kDa each.

PNA is a carbohydrate-free protein that displays specificity towards β-D-Gal(1-3)-D-GalNAc. It has potent anti-T activity and can be used to distinguish between human lymphocyte subsets. PNA has been used in tumour tissue determination for transitional mucosa malignancies. The lectin agglutinates rabbit erythrocytes at < 0.1 μg/ml after trypsin treatment of cells and its activity is inhibited by lactose and galactose.

Aladdin’s PNA lectin is provided as a white to light-yellow lyophilized powder from 10 mM NH₄HCO₃. The purity is determined by SDS-PAGE, which generates one major band at 25-27 kDa.

Benefits

• Strong anti-T activity

• Sugar specificity: β-D-Gal-(1-3)-D-GalNAc

• Agglutinates rabbit erythrocytes at < 0.1 μg/ml after trypsin treatment of the cells

• High activity

Applications

• Probe in histochemistry and immuno-histochemistry

• Binds to a broad range of receptors in human tissues

• Human lymphocyte subset studies

Specifications

ParameterDetails
AppearanceWhite to light-yellow lyophilized powder
SourcePeanuts
Molecular weight110 kDa
Sugar specificityβ-D-Gal-(1-3)-D-GalNAc
ActivityAgglutinates rabbit erythrocytes at < 0.1 μg/ml. Agglutination fully inhibited by 10 mM D-galactose
Microorganisms≤ 100 CFU/g
Shelf life≥ Three years when stored at -20°C

Directions for use

The lectin may be reconstituted with PBS or the buffer of your choice before use. Spin the vial until dissolved.

Tips and hints

Avoid repeated freezing and thawing.

Shipping and storage

The product is stable for at least 3 years from production date when stored below -20°C. May be shipped at -20°C however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than three years from production date when stored below -20°C. After reconstitution with deionized water, the solution may be stored frozen in working aliquots for up to 12 months.

规格

产品名称
花生凝集素 (PNA)
英文别名
Arachis hypogaea lectin | Lectin from Arachis hypogaea (peanut)
规格或纯度
BioReagent, 天然, Protein content ≥85%
生物活性
Agglutinates rabbit erythrocytes at < 0.1 µg/ml. Agglutination fully inhibited by 10 mM D-galatose
种属
Arachis hypogaea (peanut)
来源
天然
预测分子量
110 kDa
分子类型
蛋白质
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
10 mM NH₄HCO₃.
浓度
Protein content ≥85%
复溶
Reconstitute in PBS or the buffer of your choice before use.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
长期储存-20℃( ≥3年);复溶后建议分装,避免反复冻融。

技术文档

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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ26F0435001 分析证书 A140087
ZJ26F0435000 分析证书 A140087
ZJ26F0435002 分析证书 A140087
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