Antitumor agent-182 (Compound 12a) decreases mitochondrial membrane potential ( MMP ) and enhances ROS levels. Antitumor agent-182 arrests the cell cycle at G0/G1 phase, induces apoptosis in HeLa. Antitumor agent-182 inhibits the proliferation of HeLa, PC-3 and HCT-15 with IC 50 s of 8.83, 10.07 and 7.84 μM, respectively.