Apoptosis inducer 25 (Compound 4H) inhibits the proliferation of cell BGC-823 with IC 50 of 0.37 μM. Apoptosis inducer 25 arrests cell cycle at G2/M phase, induces apoptosis in cell BGC-823, and causes mitochondrial dysfunction. Apoptosis inducer 25 exhibits good pharmacokinetic properties in rats.