Antiproliferative agent-25 (Compound 3s4) is a selective PRMT5 inhibitor ( IC 50 : 0.11 μM). Antiproliferative agent-25 up-regulates hnRNP E1 protein level. Antiproliferative agent-25 forms H-bond interactions with SAM and E444 residue of PRMT5. Antiproliferative agent-25 has antiproliferative effects against A549 cells by inducing apoptosis and inhibiting cell migration. Antiproliferative agent-25 has high clearances with T 1/2 of only 21.8 and 4.7 min in human and rat liver microsomes.