ALK5-IN-32 (compound EX-09) is a selective ALK-5 inhibitor (10 nM< IC 50 <100 nM), inhibits TGF-β -induced SMAD signaling. ALK5-IN-32 has the potential to inhibit growth of tumour in vivo. ALK5-IN-32 can be used in study of proliferative diseases such as cancer , fibrotic diseases, and systemic sclerosis.