计算溶液所需的质量、体积或浓度。
抗酸染色液（聚集诱导发光）
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
预期用途
用于分枝杆菌等细菌的抗酸荧光染色。
检验原理
本产品利用分枝杆菌可以与AIE荧光染料结合且不易被酸性脱色液洗脱的特性。使得分枝杆菌在荧光显微镜 特定通道观察下有显著荧光，而非分枝杆菌组分则不显示或显示微弱荧光，从而分辨并检测出分枝杆菌，该染 色液可用于低倍镜下观察分枝杆菌，其形态结构清晰可辨。
产品效果
含分枝杆菌的痰液样本染色效果
样本要求
适用于痰液样本。
组分表
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使用本产品前请仔细阅读本说明书。
检验方法
1. 将待检样本涂抹在载玻片上，加热固定，形成厚薄适宜的涂膜；
2. 滴加AIE染色液直至覆盖涂膜，置于60℃加热台上染色3min（必要时可补加染色液，防止染色液蒸干）， 蒸馏水冲洗；
3. 滴加酸性脱色液直至覆盖涂膜，脱色3min，蒸馏水冲洗，盖上盖玻片；
4. 通过荧光显微镜的 B 通道（激发：460-490nm；发射：510-550nm）对涂片进行观察。分枝杆菌显 示出明亮的绿色且形态结构清晰可辨，非分枝杆菌组分不显示颜色或显示微弱的绿色。
注意事项
1. 试剂取用后盖紧试剂瓶，以防试剂挥发和污染。
2. 使用本品时应佩戴一次性手套，避免和皮肤、眼睛接触，一旦接触，立即用大量清水冲洗，如果误服或 者溅入眼睛，请及时就医。
3. 所有样本、生物试剂及实验物品都应被视为有潜在传染性，须根据当地法规按照医疗废物处理。
4. 使用本产品过程中如有问题或建议，请与厂家联系。
Intended Use
For acid - fast fluorescence staining of mycobacteria and other bacteria.
Test Principle
This product utilizes the characteristic that mycobacteria can bind to AIE fluorescent dyes and are not easily washed off by acidic decolorizing solution. As a result, mycobacteria show significant fluorescence under the observation of a specific channel of a fluorescence microscope, while non - mycobacterial groups show no or very weak fluorescence, thus distinguishing and detecting mycobacteria. This staining solution can be used to observe mycobacteria under a low - power microscope, and their morphological structure is clearly distinguishable.
Product Effect
Staining effect of sputum samples containing mycobacteria.
Sample Requirements
Suitable for sputum samples.
Test Method
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Please read this instruction manual carefully before using this product.
1.Smear the sample to be tested on a glass slide, heat and fix it to form a coating film of appropriate thickness.
2.Add AIE staining solution until the coating film is covered, place it on a 60℃ heating plate and stain for 3min (additional staining solution can be added if necessary to prevent the staining solution from drying), then rinse with distilled water.
3.Add acidic decolorizing solution until the coating film is covered, decolorize for 3min, rinse with distilled water, and cover with a cover slip.
4.Observe the smear through channel B of the fluorescence microscope (Excitation: 460 - 490nm; Emission: 510 - 550nm). Mycobacteria show bright green and their morphological structure is clearly distinguishable, while non - mycobacterial groups show no color or very weak green.
Precautions
1.Cap the reagent bottle after taking the reagent to prevent reagent volatilization and contamination.
2.When using this product, wear disposable gloves to avoid contact with skin and eyes. Once in contact, immediately rinse with a large amount of water. If swallowed or splashed into the eyes, please seek medical attention in time.
3.All samples, biological reagents and experimental materials should be regarded as potentially infectious and must be disposed of as medical waste in accordance with local regulations.
4.If there are any problems or suggestions during the use of this product, please contact the manufacturer.
规格
规格或纯度
BioReagent, 生物染色剂, 用于显微镜
稳定性与储存
2-8℃储存 （1年）；避光。
英文名称
Acid-Fast Fluorescent Staining Solution (Aggregation-induced emission, AIE)
储存条件
2-8°C储存,避光
运输条件
低温运输
Excitation(nm)
Blue Laser(488nm)
Emission(nm)
510-550nm
Ex/Em(nm)
Ex:470nm;Em:510-550nm
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ25F1028458 |
分析证书 | A1456454 |
技术文档和文章
溶液计算器
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