本品适用于干片法检测阴道分泌物，通过微生物双重荧光染色液（干片法，聚集诱导发光），可以区分出样本内的上皮细胞、白细胞、真菌和细菌。
产品特性
微生物双重荧光染色液（干片法，聚集诱导发光）是一种灵敏度高、稳定性强、适用性广的微生物染料，可用于对阴道分泌物进行涂片染色，是化学染色的理想替代品，在5min内即可获得清晰的视野成像，操作简单，结果直观。利用双重荧光染色技术，AIE复合荧光染料将样本中的上皮细胞、白细胞、线索细胞、细菌及真菌等进行染色。在荧光显微镜特殊波段激发下，被染色的微生物在黑暗背景中发出不同的荧光，形态结构清晰可辨。
实验方法
1．棉签蘸取少量新鲜样本（采集后2小时内使用）涂抹在载玻片上，再置于酒精灯火焰上方水平晃动2-3次进行固定，或用50°C加热板烤1-2min，或者室温晾干5min左右，观察时各组分不移动为宜；
2．用移液枪吸取5-10μL平衡至室温的染色液，滴加在载玻片上，加盖盖玻片，置于荧光显微镜下观察。
样本要求
适用于女性阴道分泌物样本。采集容器应清洁，一般采用生理盐水浸湿的棉拭子于阴道深部或阴道后穹隆、宫颈口等处取得样本。
注意事项
1、使用前请先短暂混匀；
2、为了您的安全和健康，请穿实验服并戴一次性手套操作；
3、本品仅适用于科研用途。
This product is suitable for detecting vaginal secretions by the dry- film method. Through the Double Fluorescent Microbial Staining Solution (Dry-film Method, Aggregation-induced emission, AIE), epithelial cells, white blood cells, fungi and bacteria in the sample can be distinguished.
Product Features
Double Fluorescent Microbial Staining Solution (Dry-film Method, Aggregation-induced emission, AIE) is a microbial dye with high sensitivity, strong stability and wide applicability. It can be used for smear staining of vaginal secretions and is an ideal substitute for chemical staining. A clear visual field image can be obtained within 5 minutes, with simple operation and intuitive results. Using the dual - fluorescence staining technology, the AIE composite fluorescent dye stains epithelial cells, white blood cells, clue cells, bacteria and fungi in the sample. Under the excitation of a special waveband of a fluorescence microscope, the stained microorganisms emit different fluorescences in the dark background, and their morphological structures are clearly distinguishable.
Experimental Method
1. Dip a cotton swab into a small amount of fresh sample (use within 2 hours after collection), smear it on a glass slide, then place it above the alcohol lamp flame and shake it horizontally 2 - 3 times for fixation, or bake it on a 50 °C hot plate for 1 - 2 minutes, or air - dry it at room temperature for about 5 minutes. It is appropriate that each component does not move during observation;
2. Use a pipette to absorb 5 - 10 μL of the staining solution equilibrated to room temperature, add it dropwise on the glass slide, cover it with a coverslip, and observe it under a fluorescence microscope.
Sample Requirements
Suitable for female vaginal secretion samples. The collection container should be clean. Generally, a cotton swab soaked in normal saline is used to obtain the sample from the deep part of the vagina, the posterior fornix of the vagina, the cervical os and other places.
Precautions
1. Please mix briefly before use;
2. For your safety and health, please wear a lab coat and disposable gloves during operation;
3. This product is only for scientific research purposes.
ZJ25F0927867
危险品化学品经营许可证（带存储）