芽孢荧光染色液是含有蓝色荧光素的荧光染液，该荧光素能与细菌产生的芽孢结合，在荧光显微镜的UV通道照射下产生蓝色荧光，相较于传统的化学荧光染色液，本品仅需一步染色即可成像，无需反复加热，且不使用孔雀绿等毒害作用大的染料，对环境影响小。
产品效果
检验方法
使用本产品前请仔细阅读本说明书。
1.制作厚度适宜的细菌涂片，自然干燥或通过50°C加热板晾干；
2.滴加一滴染色液在载玻片上，以染色液覆盖或淹没整个样本（区域）为准；
3.于50°C加热板上加热2~3min，仍残留少量染色液时，盖上盖玻片，于荧光显微镜UV通道下观察结果，芽孢被染上明亮的蓝色。
注意事项
1.使用本品时应佩戴一次性手套，避免和皮肤、眼睛接触，一旦接触，立即用大量清水冲洗，如果误服或者溅入眼睛，请及时就医。
2.供芽孢染色的培养物应控制菌龄，大部分芽孢留在菌内时为宜。
3.所有样本、生物试剂及实验物品都应被视为有潜在传染性，须根据当地法规按照医疗废物处理。
4.使用本产品过程中如有问题或建议，请与厂家联系。
The Endospore Fluorescent Staining Solution is a fluorescent staining solution containing blue fluorescein. This fluorescein can bind to spores produced by bacteria and produce blue fluorescence under the irradiation of the UV channel of a fluorescence microscope. Compared with traditional chemical fluorescent staining solutions, this product can achieve imaging with only one - step staining, without repeated heating. Moreover, it does not use dyes with high toxic effects such as malachite green, and has little impact on the environment.
Product effect
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Inspection Method
Please read this instruction manual carefully before using this product.
Prepare a bacterial smear of appropriate thickness and let it air - dry naturally or dry it on a 50 °C hot plate.
Add one drop of the staining solution onto the glass slide, making sure the staining solution covers or submerges the entire sample (area).
Heat on a 50 °C hot plate for 2 - 3 minutes. When a small amount of the staining solution remains, cover with a cover slip and observe the result under the UV channel of a fluorescence microscope. The spores will be stained bright blue.
Precautions
When using this product, wear disposable gloves to avoid contact with skin and eyes. In case of contact, immediately rinse with plenty of water. If accidentally ingested or splashed into the eyes, seek medical attention promptly.
The culture for spore staining should be controlled in terms of bacterial age, and it is appropriate when most spores remain inside the bacteria.
All samples, biological reagents and experimental items should be regarded as potentially infectious and must be disposed of as medical waste in accordance with local regulations.
If you have any questions or suggestions during the use of this product, please contact the manufacturer.
ZJ25F1028443
危险品化学品经营许可证（带存储）