ALK5-IN-8 is a potent inhibitor of TGFβRI ( ALK5 ). ALK5-IN-8 Inhibits the phosphorylation of ALK5 on its downstream signaling proteins ( Smad2 or Smad3 ) by blocking the binding of TGFβRI to ligands, thereby affecting or blocking TGF-β signaling. ALK5-IN-8 has the potential for the research of various ALK5-mediated related diseases (extracted from patent WO2021190425A1, compound 1).