BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
胺偶联试剂盒是一种专门用于将生物分子固定到生物传感器芯片表面的试剂盒。它通过化学偶联反应，将带有氨基的生物分子（如抗体、蛋白质或多肽）与传感器芯片表面的羧基基团连接起来，形成稳定的共价键。这种连接方式适用于多种生物分子，广泛用于表面等离子体共振（SPR）实验中。
本试剂盒所含试剂可完成30至50次偶联。
本试剂盒适用于Biacore 8K+、Biacore 8K、Biacore T200、Biacore S200 和 Biacore X100 系统 。
组分表
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使用说明
1. 试剂制备
a) 向EDC和NHS中各加入10.0 mL经过滤的去离子水，溶解固体。
b) 拧紧瓶盖，振荡至固体完全溶解。
c) 将EDC和NHS溶液分装成小份储存。
d) EDC和NHS分装液需在-18°C或更低温度下冷冻保存；乙醇胺可于2°C至8°C储存。
注：分装液需在两个月内使用。
2. 操作处理
a) 解冻冷冻分装液，轻轻振荡确保溶液均匀。
b) 对于配备自动进样器的Biacore系统：按软件指示将分装液分别置于自动进样器中。
c) 对于无自动进样器的Biacore系统：使用前立即将等体积EDC与NHS混合。
3. 推荐的固定流程
本方法为通过胺偶联法将配体固定至Biacore™传感器芯片提供建议，用户需根据实际情况优化反应条件。
a) 羧基活化：注入EDC/NHS（1:1）混合液6–10分钟（PEG传感器芯片为30秒。
b) 配体固定：注入配体溶液5–10分钟。注意：配体溶液需用低盐缓冲液（如pH 4.0–5.5的醋酸盐缓冲液）稀释。
c) 终止反应：注入乙醇胺6–7分钟以封闭剩余反应基团。
4. 注意事项
a) EDC与NHS试剂需现用现配，使用前新鲜混合。溶液不新鲜会降低固定效率。
b) EDC/NHS形成的酯键半衰期短，表面活化后需立即注入配体。
Amine Coupling Kit is a specialized set of reagents and materials used to covalent immobilization biomolecules (like antibodies, proteins, or peptides) carrying a primary amine group onto a carboxylated sensor surface for analysis using Surface Plasmon Resonance (SPR) biosensors.
The kit contains reagents sufficient for 30 to 50 immobilizations.
The kit can be used with All Biacore™ systems except Biacore 4000, Biacore A100 and Biacore S51.
Contents and storage
| A1507130 | Component | 1 kit | Storage |
| A1507130A | N-Hydroxysuccinimide (NHS) | 115mg | 2–8°C |
| A1507130B | 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) | 750mg | 2–8°C |
| A1507130C | 1 M Ethanolamine hydrochloride-NaOH pH 8.5 | 10.5mL | 2–8°C |
Instructions for Use
1. Reagent preparation
a) Dissolve the EDC and NHS by adding 10.0 mL of filtered, deionized water to each vial.
b) Cap vials tightly and agitate until the solids are completely dissolved.
c) Dispense the EDC and NHS solutions separately in aliquots for storage.
d) Store the aliquots of EDC and NHS frozen at -18°C or below. Ethanolamine may be stored at 2°C to 8°C.
Note: Use aliquots within two months.
2. Handling
a) Thaw frozen aliquots and agitate them gently to make sure that the solutions are homogeneous.
b) For Biacore systems with autosampler:
Place the aliquots separately in the autosampler as instructed in the software.
c) For Biacore systems without autosampler:
Mix equal volumes of EDC and NHS immediately before use.
3. Suggested immobilization procedure
This guideline provides recommendations for immobilization of ligands by amine coupling to Biacore™ sensor chips. The user needs to optimize the reaction conditions based on the actual situation.
a) Activate the surface by injecting a mixture of EDC/NHS (1:1) for 6–10 min (30 s for Sensor Chip PEG).
b) Immobilize the ligand by injecting the ligand solution for 5–10 min.
c) Deactivate excess reactive groups by injecting ethanolamine for 6–7 min.
Important considerations
1. EDC and NHS Reagent solutions should be freshly prepared and mixed shortly before use. The efficiency of the immobilization will be reduced if solutions are not fresh.
2. The EDC/NHS ester has a short half-life, so ligand should be injected shortly after activation
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0838805 |
分析证书 | A1507130 |
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