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静脉血离体后至完全凝固所需的时间即为凝血时间，它是反映内源性凝血系统各凝血因子活性的筛选实验。向全血中加入白陶土-脑磷脂混悬液，白陶土充分激活凝血因子Ⅻ、Ⅺ，启动内源性凝血系统，脑磷脂则为凝血反应提供丰富的催化表面，促进凝血过程，提高实验的敏感性，所检测的血液凝固时间即为活化凝血时间（activated clotting time，ACT）。
活化凝血时间（ACT）是内源性凝血系统敏感的筛选实验之一，该试剂盒主要用于检测人、动物血液的活化凝血时间的测定。该试剂盒仅用于科研领域，不适用于临床诊断或其他用途。
| 货号 | Component | 50T | Storage |
| A1507810 | 白陶土-脑磷脂混悬液 | 10 mL | 2-8℃ |
操作步骤
1、取内径为8mm的玻璃试管2支，分别加入白陶土-脑磷脂混悬液0.2ml。
2、静脉采血1ml，立即取下针头，沿管壁向各试管注入血液0.5ml，立即混匀并启动秒表计时，并置于37℃水浴中。
3、每隔10s轻摇试管一次，观察管内血液流动情况，直至血液凝固。记录血液凝固所需时间，即为ACT。
4、取2支试管血液凝固时间的平均值作为ACT值。
参考区间：(1.70±0.76) min
注意事项
1、所用器材应清洁干燥。试管内径要固定且一致，试管内径越大，凝血时间越长。
2、采血要求一针见血（30s内完成），最好不用扎压脉带，避免组织液和空气混入，避免标本溶血。
3、水浴温度应控制在37±0.5℃，温度过高或过低均可使ACT时间缩短，过低则ACT时间延长。
5、在肝素活化后，ACT保持在360～450s为宜；在肝素中和后ACT应小于130s。
The time required for venous blood to completely clot after being drawn from the body is known as the clotting time. It serves as a screening test reflecting the activity of coagulation factors in the intrinsic coagulation pathway. When a kaolin‑cephalin suspension is added to whole blood, kaolin fully activates coagulation factors XII and XI, thereby initiating the intrinsic coagulation system. Cephalin provides a rich catalytic surface for the coagulation reaction, promoting the clotting process and enhancing the sensitivity of the test. The measured blood clotting time under these conditions is referred to as the activated clotting time (ACT).
The activated clotting time (ACT) is one of the sensitive screening tests for the intrinsic coagulation system. This kit is primarily used for the determination of the activated clotting time in human and animal blood. It is intended for research use only and is not suitable for clinical diagnosis or other purposes.
| Product Code | Component | 50T | Storage |
| A1507810 | Kaolin‑Cephalin Suspension | 10 mL | 2-8℃ |
Procedure
1. Take two glass test tubes with an inner diameter of 8 mm and add 0.2 mL of kaolin‑cephalin suspension to each.
2. Collect 1 mL of venous blood. Immediately remove the needle and gently deliver 0.5 mL of blood along the inner wall into each test tube. Mix immediately, start a stopwatch, and place the tubes in a 37 °C water bath.
3. Gently tilt each tube every 10 seconds and observe the fluidity of the blood until clotting occurs. Record the time required for blood to clot; this is the ACT.
4. Calculate the average clotting time of the two tubes as the final ACT value.
Reference Interval: (1.70 ± 0.76) minutes
Notes
1. All equipment used should be clean and dry. The inner diameter of the tubes must be fixed and consistent, as a larger inner diameter results in a longer clotting time.
2. Blood collection should be successful on the first attempt (completed within 30 seconds). Use of a tourniquet is not recommended. Avoid contamination with tissue fluid or air, and prevent hemolysis of the specimen.
3. The water bath temperature should be controlled at 37 ± 0.5 °C. Temperatures either too high or too low can shorten the ACT, while excessively low temperatures will prolong the ACT.
4. When observing the endpoint, tilt the tube gently, each time at an angle of about 30°, to reduce the contact area between the blood and the tube wall. Observe the blood flow under bright light. The endpoint is defined as the initial stage of clotting, indicated by slowed blood flow or visible turbidity.
5. After heparin activation, the ACT should ideally be maintained between 360 and 450 seconds. Following heparin neutralization, the ACT should be less than 130 seconds.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0131576 |
分析证书 | A1507810 |
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