计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜,适用于微生物学 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
分枝杆菌的细胞壁内含有大量脂质包围在肽聚糖的外面,所以分枝杆菌一般不易着色。传统的染色方法要经过加热和延长染色时间来促使其着色。分枝杆菌中的分枝菌酸与染料一旦结合后,就很难被酸性脱色液脱色,故名抗酸染色。抗酸染色液 (改良Kinyoun冷染法) 属于冷染色液,无需加热,相对较抗酸热染液安全。其染色原理是在室温条件下,分枝菌酸不复红结合成复合物,经亚甲蓝复染后,分枝杆菌仍然为红色,而其他细菌及背景中的物质为蓝色。
产品组分及储存条件:
| A1510398 | Component | 3×50mL | 3×100mL | Storage |
| A1510398A | 改良 Kinyoun 复红染色液 | 50mL | 100mL | RT. Store in the dark. |
| A1510398B | Kinyoun 脱色液 | 50mL | 100mL | RT |
| A1510398C | 亚甲蓝染色液 | 50mL | 100mL | RT. Store in the dark. |
操作步骤(仅供参考):
1、接种环挑取待检样本,涂布于载玻片上,自然干燥。
2、滴加改良Kinyoun复红染色液染色。
3、务必加热,蒸馏水冲洗。
4、用Kinyoun脱色液脱色。
5、蒸馏水冲洗。
6、用亚甲蓝染色液染色。
7、蒸馏水冲洗。
8、轻轻吸干水分,自然干燥。
9、油镜镜检。
染色结果:
抗酸或弱抗酸菌:红色
非抗酸菌、细胞、背景:浅蓝色
注意事项:
1、每次使用后盖紧试剂瓶,以防试剂挥发和污染。
2、上述试剂均对人体有刺激性,请注意适当防护。
3、为了您的安全和健康,请穿实验服并戴一次性手套操作。
4、本产品仅供科研使用,严禁它用。
The cell wall of mycobacteria contains a large amount of lipids that encase the peptidoglycan layer, which is why mycobacteria are generally difficult to stain. Traditional staining methods require heating and extended staining time to facilitate dyeing. Once mycolic acid in mycobacteria binds to the dye, it is resistant to decolorization by acidic decolorizing solution — hence the name acid-fast staining. The Acid-fast Stain Solution (Modified Kinyoun Cold Staining Method) is a cold-type stain that does not require heating, making it relatively safer than heat-based acid-fast stain solutions. Its staining principle is as follows: at room temperature, mycolic acid combines with fuchsin to form a stable complex. After counterstaining with methylene blue, mycobacteria remain bright red, while other bacteria and background substances are stained blue.
Product Components and Storage Conditions:
| A1510398 | Component | 3×50mL | 3×100mL | Storage |
| A1510398A | Modified Kinyoun Fuchsin Stain Solution | 50mL | 100mL | RT. Store in the dark. |
| A1510398B | Kinyoun Decolorizing Solution | 50mL | 100mL | RT |
| A1510398C | Methylene Blue Stain Solution | 50mL | 100mL | RT. Store in the dark. |
Operating Procedures (For Reference Only):
1. Pick the test sample with an inoculating loop, spread it evenly on a glass slide, and air-dry naturally.
2. Add a drop of Modified Kinyoun Fuchsin Stain Solution and perform staining.
3. Heating is mandatory. Rinse the slide with distilled water.
4. Decolorize the slide with Kinyoun Decolorizing Solution.
5. Rinse with distilled water.
6. Stain the slide with Methylene Blue Stain Solution.
7. Rinse with distilled water.
8. Blot the water gently and air-dry naturally.
9. Examine the slide under an oil immersion lens.
Staining Results:
Acid-fast or weakly acid-fast bacteria: Red
Non-acid-fast bacteria, cells, and background: Light blue
Precautions:
1. Tighten the reagent bottle caps immediately after each use to prevent reagent volatilization and contamination.
2. All the above reagents are irritating to the human body; please take appropriate protective measures.
3. For your safety and health, wear a lab coat and disposable gloves during operation.
4. This product is for research use only. Any other applications are strictly prohibited.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | A1510398 | |
| 分析证书 | A1510398 |
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