阿拉丁生产的LumiDye™ 叠氮修饰的链霉亲和素 (N₃-SA)是一种即用型的点击化学偶联试剂，采用高纯度链霉亲和素与叠氮基团共价修饰而成。该产品可与末端炔烃或者DBCO修饰的抗体、寡核苷酸、聚合物或其它分子在温和条件下实现快速、高效的共价偶联。
链霉亲和素具有生物学特性，每个分子由 4 个亚基构成，能够与 4 个生物素分子紧密结合，这种强相互作用的亲和常数高达 10¹⁵mol/L ，在生物检测、分离以及靶向传递等众多领域应用。而叠氮基团具有化学活性，可参与点击化学反应，如经典的铜催化的叠氮-炔环加成反应（CuAAC），能够在温和条件下与含炔基的生物分子高效反应，形成稳定的三唑环结构。N₃-SA 融合了链霉亲和素与叠氮基团的优势，在生物分子的标记、偶联操作中，可先利用链霉亲和素与生物素化的分子特异性结合，再借助叠氮基团与炔基化分子的点击反应，实现多种生物分子的准确连接，为生物研究提供了便捷且工具，并且整个反应过程条件相对温和，对生物分子的原有活性影响较小。
使用建议
储存条件：冻干粉在2-8℃干燥、避光长期保存；复溶后溶液建议4℃保存不超过1周，或-20℃分装冻存。
推荐点击反应体系：叠氮-炔反应通常需在含CuSO₄、配体（如THPTA或TBTA）和还原剂（如抗坏血酸）的环境中进行。
典型反应条件：0.1–1 mg/mL 叠氮修饰的链霉亲和素 (N₃-SA)，炔基目标物摩尔过量2–5倍，反应在室温进行30–60分钟。
如用于细胞内标记，建议使用铜离子清除剂或采用无铜点击（SPAAC）替代。
LumiDye™ Streptavidin Azide (N₃-SA) is a ready-to-use click chemistry conjugation reagent, produced by covalent modification of high-purity streptavidin with azide groups. This product enables rapid and efficient covalent conjugation with terminal alkyne- or DBCO-modified antibodies, oligonucleotides, polymers, or other molecules under mild conditions.
Streptavidin possesses distinct biological properties: each molecule consists of four subunits capable of binding four biotin molecules with extremely high affinity, exhibiting a binding constant as high as 10¹⁵ M⁻¹, making it widely applicable in areas such as biological detection, separation, and targeted delivery. The azide group is chemically reactive and participates in click chemistry reactions, such as the classic copper-catalyzed azide-alkyne cycloaddition (CuAAC), which efficiently reacts with alkyne-containing biomolecules under mild conditions to form a stable triazole ring structure. N₃-SA combines the advantages of streptavidin and azide groups, enabling specific labeling and conjugation of biomolecules—first through streptavidin’s selective binding to biotinylated molecules, followed by the click reaction between the azide and alkyne-functionalized molecules. This allows for precise connection of various biomolecules, providing a convenient tool for biological research, with the entire process conducted under relatively mild conditions that minimally affect the original activity of biomolecules.
Usage Suggestion
Storage conditions: The lyophilized powder should be stored dry and protected from light at 2–8°C for long-term preservation. After reconstitution, the solution is recommended to be stored at 4°C for no more than 1 week, or aliquoted and frozen at –20°C.
Recommended click reaction system: Azide-alkyne reactions are typically carried out in the presence of CuSO₄, a ligand (e.g., THPTA or TBTA), and a reducing agent (e.g., ascorbic acid).
Typical reaction conditions: 0.1–1 mg/mL azide-modified streptavidin (N₃-SA), 2–5 fold molar excess of alkyne-target molecule, reaction at room temperature for 30–60 minutes.
For intracellular labeling, it is recommended to use a copper chelator or replace with copper-free click chemistry (SPAAC).
ZJ26F0535840
危险品化学品经营许可证（带存储）