Name: 无动物源类器官细胞因子套装
SKU: A1511069
Availability: InStock

概述规格储存与运输技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 技术文档和文章溶液计算器

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为什么选择此级别

BioReagent,无菌过滤级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存。超低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

背景介绍：

类器官基础套装包含在细胞培养中开发类器官模型所需的关键成分。

该包装内含以下五个小管：

无动物源性重组人Wnt-3a蛋白 (货号：rp168381)

无动物源性重组人Noggin蛋白 (货号：rp156341)

无动物源性重组人R-Spondin 1蛋白 (货号：rp169631)

无动物源性重组人FGF-10蛋白 (货号：rp145913)

无动物源性重组人EGF蛋白 (货号：rp228964)

成纤维细胞生长因子（FGF）家族成员之一的 FGF-10 具有广泛的促有丝分裂和细胞存活活性，并参与多种生物过程，包括胚胎发育、细胞生长、形态发生、组织修复、肿瘤生长和侵袭。

FGF-10 在上皮-间充质相互作用中发挥着关键作用，这对多种器官的发育至关重要。

表皮生长因子（EGF）通过与表皮生长因子受体（EGFR）结合来发挥其作用，EGFR 是一种 170 千道尔顿的蛋白激酶。EGFR 的激活会响应有毒环境刺激或 EGF 与受体结合而启动多种细胞通路。EGF 促进上皮祖细胞的快速增殖，这对于类器官的初始生长和扩张至关重要。

R-Spondin-1 是 R-Spondin 家族的一员，编码一种分泌型激活蛋白，具有两个富含半胱氨酸的、类似弗林蛋白酶的结构域和一个血小板反应蛋白 1 型结构域。

Wnt3a 是 WNT 基因家族的一部分，由结构相关的基因组成，这些基因编码分泌的信号蛋白。这些蛋白质与肿瘤发生以及包括胚胎发育期间细胞命运的调节和模式形成在内的若干发育过程有关。Wnt-3A 通过促进祖细胞增殖和分化为各种细胞类型来支持类器官的生长和形态发生，有助于复杂组织结构的形成。

组份表：

A1511069	组份	物理外观	包装1	包装2	储存	单次测试使用量
A1511069A	无动物源性重组人Wnt-3a蛋白	冻干	10 μg	50 μg	-20°C	按照需求使用
A1511069B	无动物源性重组人Noggin蛋白	冻干	10 μg	50 μg	-20°C	按照需求使用
A1511069C	无动物源性重组人R-Spondin 1蛋白	冻干	10 μg	50 μg	-20°C	按照需求使用
A1511069D	无动物源性重组人FGF-10蛋白	冻干	10 μg	50 μg	-20°C	按照需求使用
A1511069E	无动物源性重组人EGF蛋白	冻干	10 μg	50 μg	-20°C	按照需求使用

信息：

名称	种属	表达系统	标签	纯度	活性	内毒素	外观	储存缓冲液	复溶
无动物源性重组人Wnt-3a蛋白	人	HEK293	C-hFc	≥75% (SDS-PAGE)	Measured by its ability to induce Topflash reporterBioactivity in CHO-K1 cells. The ED50 for this effect is ＜30ng/ml.	<0.1 EU/μg	冻干	Lyophilized from a 0.2 um filtered solution containing PBS, pH7.4	It is recommended to redissolve in sterile deionized water.
无动物源性重组人Noggin蛋白	人	HEK293	C-His	≥90% (SDS-PAGE)	/	<1.0 EU/μg	冻干	Lyophilized from PBS, pH 7.4	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
无动物源性重组人R-Spondin 1蛋白	人	HEK293	C-His	≥95%(SDS-PAGE)	/	<1.0 EU/μg	冻干	Lyophilized from PBS, pH 7.4.	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
无动物源性重组人FGF-10	人	E. coli	No tag	≥95%(SDS-PAGE)	Measured in a cell proliferation assay using MCF‑7 human breast cancer cells in the presence of 2 μg/mL of heparin sodium. The ED50 for this effect is typically <21ng/mL.	<0.1 EU/μg	冻干	Lyophilized from a sterile solution containing 20 mM phosphate buffer, pH7.4.	Centrifuge first and redissolve with deionized water to proposal concentration.
无动物源性重组人EGF蛋白	人	E. coli	No tag	≥95%(SDS-PAGE)	Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.22 ng/mL.	<1.0 EU/μg	冻干	Lyophilized from PBS, pH 7.4	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

注意事项：

1、本产品仅作科研用途。

2、为了您的安全和健康，请穿实验服并佩戴一次性手套操作。

Background Introduction:

The Organoid Essentials Package contains the key components required for the development of organoid models in cell culture.

The package contains the following five vials:

Animal-Free Human Wnt-3a (Cat. No. rp168381)
Animal-Free Human Noggin (Cat. No. rp156341)
Animal-Free Human R-Spondin 1 (Cat. No. rp169631)
Animal-Free Human FGF-10 (Cat. No. rp145913)
Animal-Free Human EGF (Cat. No. rp228964)

FGF-10 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. FGF-10 plays a crucial role in epithelial-mesenchymal interactions, which are essential for the development of various organs.

EGF (epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli or to EGF binding to the receptor. EGF promotes the rapid proliferation of epithelial progenitor cells, which is essential for the initial growth and expansion of organoids.

R-Spondin-1 is a member of the R-spondin family and encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain.

Wnt3a, part of the WNT gene family, consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt-3A supports the growth and morphogenesis of organoids by encouraging the proliferation and differentiation of progenitor cells into various cell types, contributing to the development of complex tissue structures.

Components:

A1511069	Components	Appearance	Size 1	Size 12	Storage	Quantity Per Test
A1511069A	Animal-Free Human Wnt-3a Protein	Lyophilized	10 μg	50 μg	-20°C	Use as per requirements
A1511069B	Animal-Free Human Noggin Protein	Lyophilized	10 μg	50 μg	-20°C	Use as per requirements
A1511069C	Animal-Free Human R-Spondin Protein	Lyophilized	10 μg	50 μg	-20°C	Use as per requirements
A1511069D	Animal-Free Human FGF-10 Protein	Lyophilized	10 μg	50 μg	-20°C	Use as per requirements
A1511069E	Animal-Free Human EGF Protein	Lyophilized	10 μg	50 μg	-20°C	Use as per requirements

Information:

Name	Species	Expression System	Tag	Purity	Activity	Endotoxin	Appearance	Storage	Reconstitution
Animal-Free Human Wnt-3a Protein	Human	HEK293	C-hFc	≥75% (SDS-PAGE)	Measured by its ability to induce Topflash reporterBioactivity in CHO-K1 cells. The ED50 for this effect is ＜30ng/ml.	<0.1 EU/μg	Lyophilized	Lyophilized from a 0.2 um filtered solution containing PBS, pH7.4	It is recommended to redissolve in sterile deionized water.
Animal-Free Human Noggin Protein	Human	HEK293	C-His	≥90% (SDS-PAGE)	/	<1.0 EU/μg	Lyophilized	Lyophilized from PBS, pH 7.4.	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Animal-Free Human R- Spondin 1 Protein	Human	HEK293	C-His	≥95%(SDS-PAGE)	/	<1.0 EU/μg	Lyophilized	Lyophilized from PBS, pH 7.4.	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Animal-Free Human FGF-10 Protein	Human	E. coli	No tag	≥95%(SDS-PAGE)	Measured in a cell proliferation assay using MCF‑7 human breast cancer cells in the presence of 2 μg/mL of heparin sodium. The ED50 for this effect is typically <21ng/mL.	<0.1 EU/μg	Lyophilized	Lyophilized from a sterile solution containing 20 mM phosphate buffer, pH7.4.	Centrifuge first and redissolve with deionized water to proposal concentration.
Animal-Free Human EGF Protein	Human	E. coli	No tag	≥95%(SDS-PAGE)	Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.22 ng/mL.	<1.0 EU/μg	Lyophilized	Lyophilized from PBS, pH 7.4.	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Notes:

1. This product is for research use only.

2. For your safety and health, please wear a lab coat and disposable gloves during operation.

规格

别名

无动物源类器官套装

英文别名

Animal-free Organoid Cytokine Set

规格或纯度

无菌过滤, BioReagent

英文名称

Animal-free Organoid Cytokine Package

应用

Biochemical assay, Cell Culture, Functional Assay, Functional Studies

储存与运输

储存条件

-20°C储存

运输条件

超低温运输

稳定性与储存

A1511069A: store at -20°C long term (36 months). A1511069B: store at -20°C long term (12 months). A1511069C: store at -20°C long term (12 months). A1511069D: store at -20°C long term (12 months). A1511069E: store at -20°C long term (12 months). -20~-80℃ 存

组分与储存

A1511069	组份	物理外观	包装1	包装2	储存	单次测试使用量
A1511069A	无动物源性重组人Wnt-3a蛋白	冻干	10 μg	50 μg	-20°C	按照需求使用
A1511069B	无动物源性重组人Noggin蛋白	冻干	10 μg	50 μg	-20°C	按照需求使用
A1511069C	无动物源性重组人R-Spondin 1蛋白	冻干	10 μg	50 μg	-20°C	按照需求使用
A1511069D	无动物源性重组人FGF-10蛋白	冻干	10 μg	50 μg	-20°C	按照需求使用
A1511069E	无动物源性重组人EGF蛋白	冻干	10 μg	50 μg	-20°C	按照需求使用

技术文档

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批次质量数据。输入批号以获取对应 COA。

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该级别的完整质量属性和验收标准。

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高级数据

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C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

技术文档和文章

a1511069_无动物源类器官基础套装.pdf

a1511069_animal-free organoid cytokine package.pdf

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无动物源类器官细胞因子套装

储存与运输

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文献证明

概述

规格

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