无动物源鼠源造血干细胞扩增细胞因子套装 (IL-6)

Name: 无动物源鼠源造血干细胞扩增细胞因子套装 (IL-6)
SKU: A1511074
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货号: A1511074

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概述

鼠源造血干细胞扩增细胞因子套装包含重组 Flt3 配体、干细胞因子（SCF）、血小板生成素（TPO）和白介素 - 6（IL - 6），这些是体外培养造血干细胞（HSCs）时所必需的关键生长因子和细胞因子。这些因子支持造血干细胞的增殖、存活和分化。

该包装内含以下4个小管：

无动物源重组小鼠FLT-3 Ligand蛋白 (货号：rp153982)

无动物源重组小鼠SCF蛋白 (货号：rp154627)

无动物源重组小鼠Thrombopoietin蛋白 (货号：rp154717)

无动物源重组小鼠IL-6蛋白 (货号：rp154238)

Flt3 配体是一种生长因子，可调节早期造血细胞的增殖。它本身并不能刺激早期造血细胞的增殖，但能与其他集落刺激因子和白细胞介素协同作用，诱导细胞生长和分化。

干细胞因子（SCF）是一种造血生长因子，通过与 c-Kit 受体信号传导发挥其活性。SCF 和 c-Kit 对于向黑素细胞和生殖细胞谱系分化的造血细胞的存活、增殖和分化至关重要。人源的 SCF 对小鼠细胞活性较低，而小鼠和大鼠的 SCF 对人细胞则具有完全活性。

血小板生成素（TPO）是一种在肝脏、肾脏和骨骼肌中产生的特异性生长因子。它能刺激巨核细胞的增殖和成熟，并在体内促进血小板循环水平的升高。

白细胞介素 - 6 是一种多效性细胞因子，可刺激 B 细胞分化和抗体生成，与白细胞介素 - 3 协同作用促进巨核细胞发育和血小板生成，诱导肝脏急性期蛋白表达，并调节骨代谢。白细胞介素 - 6 通过由白细胞介素 - 6 受体（IL - 6R）和 gp130 两条链组成的白细胞介素 - 6 受体系统进行信号传导。重组人白细胞介素 - 6 是一种 20.9 千道尔顿的蛋白质，包含 184 个氨基酸残基。

信息：

名称	种属	表达系统	标签	纯度	活性	内毒素	外观	储存缓冲液	复溶
重组小鼠FLT-3 Ligand蛋白	小鼠	HEK293	C-hFc & His	≥90%(SDS-PAGE)	Immobilized Recombinant Mouse FLT3, His Tag at 3 μg/mL (100 μL/well) can bind Recombinant Mouse Flt-3 Ligand, The EC50 of Mouse Flt-3 Ligand is 36 ng/mL.	<0.1 EU/μg	冻干	Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
重组小鼠SCF蛋白	小鼠	HEK293	C-His	≥95%(SDS-PAGE)	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.5-1.0 ng/mL, corresponding to a specific activity of 1.0×10^6-2.0×10^6 units/mg.	<0.1 EU/μg	冻干	Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4.	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
重组小鼠Thrombopoietin蛋白	小鼠	HEK293	N-His	≥90%(SDS-PAGE)	Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED₅₀ for this effect is typically 0.35 ng/mL.	<1.0 EU/μg	冻干	Lyophilized from sterile 10mM PBS, 5% Trehalose, 5% Mannitol, 0.01% Tween-80, pH7.4.	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25mg/mL.
重组小鼠IL-6蛋白	小鼠	HEK293	C-His	≥95%(SDS-PAGE)	Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 0.07-0.28 ng/mL, corresponding to a specific activity of 3.57×10^6 ~ 1.43×10^7 units/mg.	<0.1 EU/μg	冻干	Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4.	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.

注意事项：

1、本产品仅作科研用途。

2、为了您的安全和健康，请穿实验服并佩戴一次性手套操作。

The Human Hematopoietic Stem Cell Expansion Cytokine Package contains recombinant Flt3 ligand, stem cell factor (SCF), thrombopoietin (TPO), and interleukin-6 (IL-6), critical growth factors and cytokines use in ex vivo culture of hematopoietic stem cells (HSCs). These factors support the proliferation, survival, and differentiation of HSCs.

The package contains the following four vials:

Animal-Free Recombinant Mouse FLT-3 Ligand Protein (Cat. No. rp153982)
Animal-Free Recombinant Mouse SCF Protein (Cat. No. rp154627)
Animal-Free Recombinant Mouse Thrombopoietin Protein (Cat. No. rp154717)
Animal-Free Recombinant Mouse IL-6 Protein (Cat. No. rp154238)

Flt3 ligand is a growth factor that regulates proliferation of early hematopoietic cells. By itself it does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation.

SCF is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation, and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells.

TPO is a lineage-specific growth factor produced in the liver, kidney, and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes and promotes increased circulating levels of platelets in vivo.

IL-6 is a pleiotropic cytokine that stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6R and gp130. Recombinant Human IL-6 is a 20.9 kDa protein containing 184 amino acid residues

Information:

Name	Species	Expression System	Tag	Purity	Activity	Endotoxin	Appearance	Storage	Reconstitution
Animal-Free Recombinant Mouse FLT-3 Ligand Protein	Mouse	HEK293	C-hFc & His	≥90%(SDS-PAGE)	Immobilized Recombinant Mouse FLT3, His Tag at 3 μg/mL (100 μL/well) can bind Recombinant Mouse Flt-3 Ligand, The EC50 of Mouse Flt-3 Ligand is 36 ng/mL.	<0.1 EU/μg	Lyophilized	Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
Animal-Free Recombinant Mouse SCF Protein	Mouse	HEK293	C-His	≥95%(SDS-PAGE)	Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.5-1.0 ng/mL, corresponding to a specific activity of 1.0×10^6-2.0×10^6 units/mg.	<0.1 EU/μg	Lyophilized	Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4.	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
Animal-Free Recombinant Mouse Thrombopoietin Protein	Mouse	HEK293	N-His	≥90%(SDS-PAGE)	Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED₅₀ for this effect is typically 0.35 ng/mL.	<1.0 EU/μg	Lyophilized	Lyophilized from sterile 10mM PBS, 5% Trehalose, 5% Mannitol, 0.01% Tween-80, pH7.4.	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25mg/mL.
Animal-Free Recombinant Mouse IL-6 Protein	Mouse	HEK293	C-His	≥95%(SDS-PAGE)	Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 0.07-0.28 ng/mL, corresponding to a specific activity of 3.57×10^6 ~ 1.43×10^7 units/mg.	<0.1 EU/μg	Lyophilized	Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4.	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.

Notes:

1. This product is for research use only.

2. For your safety and health, please wear a lab coat and disposable gloves during operation.

规格

别名

无动物源鼠源造血干细胞扩增细胞因子套装

英文别名

Animal-free Murine Hematopoietic Stem Cell Expansion Cytokine Package (IL-6)

规格或纯度

无菌过滤, BioReagent

英文名称

Animal-free Mouse Hematopoietic Stem Cell Expansion Cytokine Package (IL-6)

应用

Biochemical assay, Cell Culture, Functional Assay

储存与运输

储存条件

-20°C储存

运输条件

超低温运输

稳定性与储存

-20~-80℃ 存储12个月；复溶后建议分装。避免反复冻融。

组分与储存

H1511074	Components	Appearance	Size 1	Size 2	Size 3	Storage	Quantity Per Test
H1511074A	无动物源重组小鼠FLT-3 Ligand蛋白	冻干	10 μg	50 μg	100 μg	-20°C	按照需求使用
H1511074B	无动物源重组小鼠SCF蛋白	冻干	10 μg	50 μg	100 μg	-20°C	按照需求使用
H1511074C	无动物源重组小鼠Thrombopoietin蛋白	冻干	10 μg	50 μg	100 μg	-20°C	按照需求使用
H1511074D	无动物源重组小鼠IL-6蛋白	冻干	10 μg	50 μg	100 μg	-20°C	按照需求使用

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技术文档和文章

a1511074_无动物源鼠源造血干细胞扩增细胞因子套装.pdf

a1511074_animal-free mouse hematopoietic stem cell expansion cytokine package.pdf

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无动物源鼠源造血干细胞扩增细胞因子套装 (IL-6)

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