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无动物源鼠源树突状细胞因子套装
有货
为什么选择此级别
BioReagent,无菌过滤 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
鼠源树突状细胞细胞因子套装包含 IL-4 和 GM-CSF，这是体外培养鼠源树突状细胞所需的关键成分。重组鼠源 IL-4 是一种在体外分化和成熟鼠源树突状细胞过程中必不可少的细胞因子。IL-4 蛋白，特别是重组 IL-4，能促进单核细胞等前体细胞向树突状细胞的发育。鼠源 IL-4 与包括 GM-CSF（粒细胞 - 巨噬细胞集落刺激因子）在内的其他因子协同作用，推动分化过程。通常，单核细胞在重组 IL-4 和 GM-CSF 的存在下培养，以生成未成熟的树突状细胞，然后可通过额外的刺激使其进一步成熟。
该包装内含以下2个小瓶：
无动物源重组小鼠白介素4蛋白（货号： rp154228）
无动物源重组小鼠粒细胞巨噬细胞集落刺激因子蛋白（货号： rp154039）
白细胞介素 - 4 是一种多效性细胞因子，可调节多种 T 细胞和 B 细胞反应，包括细胞增殖、存活和基因表达。由肥大细胞、T 细胞和骨髓基质细胞产生，白细胞介素 - 4 调节初始 CD4+ T 细胞向辅助性 Th2 细胞的分化。
GM-CSF 是一种造血生长因子，它能刺激中性粒细胞和巨噬细胞的发育，并促进早期红系巨核细胞和嗜酸性祖细胞的增殖与发育。
组份表：
| A1511077 | 组件 | 物理外观 | 包装1 | 包装2 | 储存 | 单次测试使用量 |
| H1511077A | 无动物源重组小鼠IL-4蛋白 | 冻干 | 10 μg | 50 μg | -20°C | 按照需求使用 |
| H1511077B | 无动物源重组鼠GM-CSF蛋白 | 冻干 | 10 μg | 50 μg | -20°C | 按照需求使用 |
信息：
| 名称 | 种属 | 表达系统 | 标签 | 纯度 | 活性 | 内毒素 | 外观 | 储存缓冲液 | 复溶 |
| 无动物源重组小鼠IL-4蛋白 | 小鼠 | HEK293 | C-His | ≥95% (SDS-PAGE) | Measured in a cell proliferation assay using assay using CTLL‑2 mouse cytotoxic T cells. The ED₅₀ for this effect is typically <1.8ng/mL. | <1.0 EU/μg | 冻干(Lyophilized) | Lyophilized from 10mM PBS, pH 7.4 | Reconstitute in sterile H2O at not less than 100µg/ml, which can then be further diluted in other aqueous solutions. |
| 无动物源重组小鼠GM-CSF蛋白 | 小鼠 | 293F | N-His | ≥95% (SDS-PAGE) | Measured in a cell proliferation assay using FDC-P1 cells. The ED50 for this effect is typically 0.04-0.17 ng/mL, corresponding to a specific activity of 5.88×10^6~2.5×10^7 units/mg | <0.1 EU/μg | 冻干(Lyophilized) | Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4. | Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml. |
注意事项：
1、本产品仅作科研用途。
2、为了您的安全和健康，请穿实验服并佩戴一次性手套操作。
The Murine Dendritic Cell Cytokine Package contains IL-4 and GM-CSF, key components required for the culturing of murine dendritic cells. Recombinant murine IL-4 is an essential cytokine used in the differentiation and maturation of murine dendritic cell in vitro. The IL-4 protein, specifically recombinant IL-4, promotes the development of dendritic cells from precursor cells such as monocytes. Murine IL-4 acts synergistically with other factors, including GM-CSF (granulocyte-macrophage colony stimulating factor), to drive the differentiation process. Typically, monocytes are cultured in the presence of recombinant IL-4 and GM-CSF to yield immature dendritic cells, which can then be futher matured by additional stimuli.
The package contains the following two vials:
Animal-Free Recombinant Mouse IL-4 Protein（Cat. No. rp154228）
Animal-Free Recombinant Mouse GM-CSF Protein （Cat. No. rp154039）
IL-4 is a pleiotropic cytokine that regulates diverse T- and B-cell responses including cell proliferation, survival, and gene expression. Produced by mast cells, T cells, and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells.
GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells.
Components：
| A1511077 | Components | Appearance | Size 1 | Size 2 | Storage | Quantity Per Test |
| A1511077A | Animal-Free Recombinant Mouse IL-4 Protein | Lyophilized | 10 μg | 50 μg | -20°C | Use as per requirements |
| A1511077B | Animal-Free Recombinant Mouse GM-CSF Protein | Lyophilized | 10 μg | 50 μg | -20°C | Use as per requirements |
Information:
| Name | Species | Expression System | Tag | Purity | Activity | Endotoxin | Appearance | Storage | Reconstitution |
| Animal-Free Recombinant Mouse IL-4 Protein | Mouse | HEK293 | C-His | ≥95% (SDS-PAGE) | Measured in a cell proliferation assay using assay using CTLL‑2 mouse cytotoxic T cells. The ED₅₀ for this effect is typically <1.8ng/mL. | <1.0 EU/μg | Lyophilized | Lyophilized from 10mM PBS, pH 7.4 | Reconstitute in sterile H2O at not less than 100µg/ml, which can then be further diluted in other aqueous solutions. |
| Animal-Free Recombinant Mouse GM-CSF Protein | Mouse | 293F | N-His | ≥95% (SDS-PAGE) | Measured in a cell proliferation assay using FDC-P1 cells. The ED50 for this effect is typically 0.04-0.17 ng/mL, corresponding to a specific activity of 5.88×10^6~2.5×10^7 units/mg. | <0.1 EU/μg | Lyophilized | Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4. | Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml. |
Notes:
1. This product is for research use only.
2. For your safety and health, please wear a lab coat and disposable gloves during operation.
规格
别名
鼠源树突状细胞因子套装
英文别名
Murine Dendritic Cell Cytokine Package
规格或纯度
无菌过滤, BioReagent
英文名称
Animal-free Mouse Dendritic Cell Cytokine Package
应用
Biochemical assay, Cell Culture, Functional Assay, Functional Studies
储存与运输
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20~-80℃ 存储12个月；复溶后建议分装。避免反复冻融。
组分与储存
| A1511077 | 组件 | 物理外观 | 包装1 | 包装2 | 储存 | 单次测试使用量 |
| A1511077A | 无动物源重组小鼠IL-4蛋白 | 冻干 | 10 μg | 50 μg | -20°C | 按照需求使用 |
| A1511077B | 无动物源重组鼠GM-CSF蛋白 | 冻干 | 10 μg | 50 μg | -20°C | 按照需求使用 |
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