BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
总蛋白(Total Protein,TP)由白蛋白和球蛋白组成，检测白蛋白的方法有双缩脲法、色氨酸法、染料结合法，其中检测白蛋白的染料结合法可采用溴甲酚绿或溴甲酚紫染料结合，上述染料对白蛋白具有高度的亲和力，通常监测染料与白蛋白结合的初速率，该速率与样品中白蛋白浓度成正比。 该试剂盒检测原理是在酸性环境下，白蛋白分子带正电荷，与带负电荷的溴甲酚紫(Bromocresol purple，BCP)结合生成绿色复合物，在603nm处有吸收波，该复合物的吸光度与白蛋白浓度成正比，与同样处理的白蛋白标准比较，求得待测样品中白蛋白浓度，可用于人血清样本中的白蛋白含量测定，该法操作简单、方法特异，既可手工操作，又可采用自动分析仪检测，对血清清蛋白的特异性比BCG法(溴甲酚绿法)要好，不易受时间和温度变化的影响。该产品仅用于科研领域，不适用于临床诊断或其他用途。
Total Protein (TP) is composed of albumin and globulin. Methods for detecting albumin include the biuret method, the tryptophan method, and dye-binding methods. Among these, the dye-binding method for albumin detection can employ bromocresol green or bromocresol purple dyes, which have a high affinity for albumin. Typically, the initial rate of dye binding to albumin is monitored, and this rate is proportional to the albumin concentration in the sample. The detection principle of this kit is as follows: In an acidic environment, albumin molecules carry a positive charge and bind with negatively charged bromocresol purple (BCP) to form a green complex. This complex has an absorption peak at 603 nm. The absorbance of the complex is proportional to the albumin concentration. By comparing with an albumin standard treated under the same conditions, the albumin concentration in the test sample can be determined. This kit is suitable for measuring albumin content in human serum samples. The method is simple to operate and specific. It can be performed manually or adapted for use with automated analyzers. It offers better specificity for serum albumin compared to the BCG (bromocresol green) method and is less susceptible to variations in time and temperature. This product is for research use only and is not intended for use in diagnostic procedures.
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