Amylose is a polysaccharide chain composed of D-glucose units linked by α-(1,4)-glycosidic bonds. The determination of its content is of significant importance for evaluating the nutritional value of food and investigating sugar metabolism in plants. This kit provides a simple, convenient, and rapid method for amylose content determination, suitable for plant samples. The principle involves using 80% ethanol to separate soluble sugars from starch in the sample, where amylose forms a complex with iodine that exhibits an absorption peak at 620 nm.