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天冬酰胺酶（EC 3.5.1.1，ASNase）是一种酰胺水解酶，能够将L-天冬酰胺脱去氨基生成L-天冬氨酸和氨。该酶具有抗肿瘤活性，在食品和医药等领域应用十分广泛。 天冬酰胺酶（ASNase）催化天冬酰胺水解成天冬氨酸和氨，利用氨在强碱的环境下与次氯酸盐和苯酚作用，生成水溶性染料靛酚蓝，溶液颜色稳定。其在630nm处有特征吸收峰，通过检测氨增加的速率，即可计算该酶活性大小。
Asparaginase (EC 3.5.1.1, ASNase) is an amidohydrolase that catalyzes the deamination of L-asparagine to produce L-aspartate and ammonia. This enzyme exhibits anti-tumor activity and is widely utilized in various fields such as the food and pharmaceutical industries. Asparaginase (ASNase) catalyzes the hydrolysis of asparagine to aspartic acid and ammonia. Under strong alkaline conditions, the ammonia reacts with hypochlorite and phenol to form a stable, water-soluble blue indophenol compound. This compound has a characteristic absorption peak at 630 nm, and the enzyme activity can be calculated by measuring the rate of increase in ammonia production.
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