计算溶液所需的质量、体积或浓度。
花青素还原酶(Anthocyanidin reductase, ANR)是原花青素生物合成途径中的关键酶之一,可使花色素转变为相应的顺式黄烷-3-醇,对植物体内类黄酮类物质的合成、花色苷的积累,具有重要作用。本试剂盒可检测生物体内 ANR 活性,其原理是:ANR 可催化 NADPH 和花色素转化为黄烷-3-醇和 NADP⁺,NADPH 在 340 nm 有吸收峰,而 NADP⁺ 没有;通过测定 340 nm 光吸收下降速率,计算 ANR 活性。
Anthocyanidin reductase (ANR) is one of the key enzymes in the proanthocyanidin biosynthesis pathway, catalyzing the conversion of anthocyanidins into their corresponding cis-flavan-3-ols. It plays a crucial role in the synthesis of flavonoid compounds and the accumulation of anthocyanins in plants. This kit enables the detection of ANR activity in biological systems. The principle is based on ANR catalyzing the conversion of NADPH and anthocyanidins into flavan-3-ols and NADP⁺. NADPH exhibits an absorption peak at 340 nm, while NADP⁺ does not. The activity of ANR is calculated by measuring the rate of decrease in absorbance at 340 nm.