计算溶液所需的质量、体积或浓度。
花青素还原酶是黄酮合成途径中的关键酶,在植物体内起非常重要的调控作用,对花青素还原酶的调控机制研究有利于从基因水平改变植物的品质。 测定原理:花青素还原酶在NADPH存在的条件下作用于飞燕草色素转变为表没食子儿茶素和NADP⁺,使反应体系在340nm处的吸光值下降,吸光值下降速率反应了花青素还原酶的活性。
Anthocyanidin reductase is a key enzyme in the flavonoid biosynthesis pathway and plays a very important regulatory role in plants. Studying the regulatory mechanism of anthocyanidin reductase is beneficial for improving plant quality at the genetic level. Determination principle: Anthocyanidin reductase acts on delphinidin in the presence of NADPH, converting it to epigallocatechin and NADP⁺, which causes a decrease in absorbance at 340 nm in the reaction system. The rate of decrease in absorbance reflects the activity of anthocyanidin reductase.