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碱性蛋白酶（AKP）是指在碱性条件下催化蛋白质肽键水解的酶类，属于丝氨酸蛋白酶。此外，该酶还能够水解酯键、酰胺键，具有转酯及转肽的功能。该酶是主要工业用酶之一，广泛应用于制药、丝绸、食品、制革等行业。在碱性条件下，AKP水解酪蛋白生成酪氨酸，酪氨酸还原磷钼酸生成钨蓝；钨蓝在 680 nm 有特征吸收峰，测定 680 nm 吸光度增加速率，从而计算AKP活性。
Alkaline Protease (AKP) refers to a class of enzymes that catalyze the hydrolysis of peptide bonds in proteins under alkaline conditions and belongs to the serine protease family. In addition, this enzyme can also hydrolyze ester bonds and amide bonds, and exhibits transesterification and transpeptidation activities. It is one of the major industrial enzymes and is widely used in industries such as pharmaceuticals, silk degumming, food processing, and leather processing. Under alkaline conditions, AKP hydrolyzes casein to produce tyrosine, which reduces phosphomolybdic acid to form tungsten blue. Tungsten blue has a characteristic absorption peak at 680 nm. AKP activity is calculated by measuring the rate of increase in absorbance at 680 nm.
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