计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
顺乌头酸酶(aconitase, ACO),三羧酸循环中的酶,催化柠檬酸转变为异柠檬酸。柠檬酸本身不易氧化,在顺乌头酸酶作用下,通过脱水与加水反应,使羟基由β碳原子转移到α碳原子上,生成易于脱氢氧化的异柠檬酸,为进一步的氧化脱羧反应作准备。该产品提供了一种简单、方便、快速的 ACO 活性检测方法,适用于检测动物组织、植物组织、细胞、血清(浆)等样本。其原理是 ACO 催化柠檬酸转化成异柠檬酸,异柠檬酸氧化脱羧将NAD⁺还原生成NADH,导致 340 nm 处光吸收上升。
Aconitase (ACO) is an enzyme in the tricarboxylic acid cycle that catalyzes the conversion of citrate to isocitrate. Citrate itself is not easily oxidized; under the action of aconitase, through dehydration and hydration reactions, the hydroxyl group is transferred from the β-carbon atom to the α-carbon atom, generating isocitrate which is readily dehydrogenated and oxidized, preparing for further oxidative decarboxylation reactions. This product provides a simple, convenient, and rapid method for assaying ACO activity, suitable for detecting samples such as animal tissues, plant tissues, cells, and serum (plasma). Its principle is that ACO catalyzes the conversion of citrate to isocitrate. Isocitrate is then oxidatively decarboxylated, reducing NAD⁺ to generate NADH, resulting in an increase in absorbance at 340 nm.