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顺乌头酸酶（aconitase），三羧酸循环中的酶，催化柠檬酸转变为异柠檬酸。柠檬酸本身不易氧化，在顺乌头酸酶作用下，通过脱水与加水反应，使羟基由β碳原子转移到α碳原子上，生成易于脱氢氧化的异柠檬酸，为进一步的氧化脱羧反应作准备。 测定原理：ACO催化柠檬酸转化成异柠檬酸，异柠檬酸氧化脱羧将NAD⁺还原生成NADH，导致340nm处光吸收上升。
Aconitase (aconitate hydratase) is an enzyme in the tricarboxylic acid (TCA) cycle that catalyzes the conversion of citrate to isocitrate. Citrate itself is not readily oxidized; under the action of aconitase, through dehydration and hydration reactions, the hydroxyl group is transferred from the β-carbon to the α-carbon, generating isocitrate, which is easily dehydrogenated and oxidized, thereby preparing for subsequent oxidative decarboxylation reactions. Determination Principle: Aconitase (ACO) catalyzes the conversion of citrate to isocitrate. Isocitrate is then oxidatively decarboxylated, reducing NAD⁺ to NADH, resulting in an increase in absorbance at 340 nm.
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