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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
腺苷二磷酸葡萄糖焦磷酸化酶(ADP-glucose pyrophosphorylase,AGP,EC 2.7.7.21)主要存在于植物中,催化葡萄糖-1-磷酸与ATP 反应生成淀粉合成的直接前体 ADPG,是植物淀粉生物合成的主要限速步骤。该产品可检测植物组织样本。在该试剂盒中,AGP 催化的逆向反应生成 G1P,在反应体系中添加的磷酸己糖变位酶和 6-磷酸葡萄糖脱氢酶依次催化生成 6-磷酸葡萄糖酸和 NADPH,340 nm 下测定 NADPH 增加速率,即可计算AGP活性。
ADP-glucose pyrophosphorylase (AGP, EC 2.7.7.21) is primarily found in plants and catalyzes the reaction between glucose-1-phosphate and ATP to form ADPG, the direct precursor for starch synthesis. This step serves as the major rate-limiting step in plant starch biosynthesis. This product is designed for detecting plant tissue samples. In this assay kit, the reverse reaction catalyzed by AGP generates G1P, which is then sequentially converted by phosphoglucomutase and glucose-6-phosphate dehydrogenase, added to the reaction system, to produce 6-phosphogluconate and NADPH. By measuring the rate of NADPH increase at 340 nm, AGP activity can be calculated.