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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
细胞色素P450酶是一组在外源物质代谢中,尤其是药物和毒物,具有重要作用的酶系。AND作为P450酶系的重要一员,相当于CYP3A4亚型,与药物的去甲基化反应密切相关。 测定原理:AND催化氨基比林释放甲醛,通过Nash比色法测定甲醛含量,即可计算出AND活性。
Cytochrome P450 enzymes are a group of enzymes that play an important role in the metabolism of xenobiotics, particularly drugs and toxins. As a key member of the P450 enzyme system, AND (aminopyrine N-demethylase) corresponds to the CYP3A4 isoform and is closely involved in the demethylation of drugs. Determination Principle: AND catalyzes the release of formaldehyde from aminopyrine. The formaldehyde content is determined using the Nash colorimetric method, allowing the calculation of AND activity.