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ABTS在氧化剂作用下被氧化为稳定的蓝绿色阳离子ABTS自由基，在405 nm或734 nm处具有特征吸收峰，抗氧化物存在时会抑制ABTS自由基的生成使反应体系褪色，405 nm处吸光值随之下降，在一定范围内吸光值的变化与自由基被清除的程度成正比，通过吸光值的下降程度即可表征样本的ABTS自由基清除能力，并提供Trolox作为阳性对照量化抗氧化物质的清除能力。
ABTS is oxidized by oxidizing agents to form a stable blue-green cation radical (ABTS⁺·), which exhibits characteristic absorbance maxima at 405 nm or 734 nm. In the presence of antioxidants, the generation of ABTS radicals is inhibited, resulting in decolorization of the reaction system and a consequent decrease in absorbance at 405 nm. Within a certain range, the change in absorbance is proportional to the extent of radical scavenging. Thus, the ABTS radical scavenging capacity of a sample can be characterized by the decrease in absorbance. Trolox is provided as a positive control to quantify the scavenging capacity of antioxidant substances.
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