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三磷酸腺苷（ATP）是生物体内能量转换的最基本载体和最直接的能量来源，测定ATP含量并计算能荷，可有效反映机体的能量代谢状态。本试剂盒采用偶联酶法测定ATP含量：在己糖激酶（HK）和6-磷酸葡萄糖脱氢酶（G6PDH）的联合催化下，ATP水解驱动葡萄糖磷酸化生成6-磷酸葡萄糖，后者进一步被G6PDH氧化，同时将NADP⁺还原为NADPH。通过监测340 nm处NADPH特征吸收峰的增量，在底物饱和条件下，单位时间内OD₃₄₀的初始线性增幅（ΔOD₃₄₀/min） 与ATP含量呈正比，据此即可精准计算样品中的ATP浓度。
Adenosine triphosphate (ATP) serves as the most fundamental carrier and the most direct source of energy in biological energy conversion. Determination of ATP content and calculation of energy charge can effectively reflect the energy metabolic status of organisms. This kit employs a coupled enzymatic method for ATP quantification: Under the combined catalysis of hexokinase (HK) and glucose-6-phosphate dehydrogenase (G6PDH), ATP hydrolysis drives the phosphorylation of glucose to glucose-6-phosphate, which is subsequently oxidized by G6PDH while reducing NADP⁺ to NADPH. By monitoring the increase in the characteristic absorbance peak of NADPH at 340 nm, under substrate-saturated conditions, the initial linear increase in OD₃₄₀ per unit time (ΔOD₃₄₀/min) is proportional to the ATP concentration. Accordingly, the ATP concentration in the sample can be precisely calculated.
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