BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
花色苷是一类易溶于极性溶剂的天然色素，属于黄酮类化合物，广泛存在于植物的根、茎、叶、花和果实中，使其呈现由红到紫等不同颜色，是植物主要的呈色物质。本试剂盒采用pH示差法测定花色苷含量：在pH 1.0时，花色苷于530 nm处具有最大吸收峰；而在pH 4.5时，花色苷转变为无色的查尔酮形式，在530 nm处无吸收峰。利用这一特性，分别测定不同pH条件下530 nm和700 nm处的吸光度值，通过计算即可定量样品中花色苷含量。pH示差法能够减少溶液pH和溶剂差异的影响，同时排除其他非花色苷类物质对检测结果的干扰。
Anthocyanins are a class of natural pigments that are readily soluble in polar solvents and belong to the flavonoid compounds. They are widely distributed in the roots, stems, leaves, flowers, and fruits of plants, imparting a range of colors from red to purple, and are the primary pigments responsible for plant coloration. This kit employs the pH differential method for the determination of anthocyanin content. At pH 1.0, anthocyanins exhibit a maximum absorption peak at 530 nm; whereas at pH 4.5, they are converted to the colorless chalcone form and show no absorption at 530 nm. Utilizing this property, the absorbance values at 530 nm and 700 nm are measured under the two pH conditions, and the anthocyanin content in the sample can be quantitatively calculated. The pH differential method minimizes the effects of variations in solution pH and solvent composition, and effectively eliminates interference from other non‑anthocyanin substances in the detection results.
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