计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
木聚糖酶(EC 3.2.1.8)主要由微生物产生,能催化水解木聚糖,也称为戊聚糖酶或半纤维素酶。该酶可分解酿造或饲料工业中的原料细胞壁及β-葡聚糖,降低酿造过程中物料的粘度,促进有效物质的释放;同时可降低饲料中的非淀粉多糖,促进营养物质吸收利用,因此广泛应用于酿造和饲料工业。碱性木聚糖酶(BAX)一般分离自最适生长pH为9–11的微生物。 本试剂盒的测定原理为:BAX在碱性环境中催化木聚糖降解为还原性寡糖和单糖;还原糖在沸水浴条件下与3,5-二硝基水杨酸(DNS)发生显色反应,生成在540 nm处具有特征吸收峰的有色产物。反应液颜色的深浅与酶解产生的还原糖量成正比,通过测定540 nm处吸光度的增加速率,即可计算BAX的酶活力。
Xylanase (EC 3.2.1.8) is mainly produced by microorganisms and catalyzes the hydrolysis of xylan. It is also known as pentosanase or hemicellulase. This enzyme can decompose cell walls and β‑glucan in raw materials used in the brewing and feed industries, thereby reducing the viscosity of the mash during brewing and promoting the release of active substances. Meanwhile, it reduces non‑starch polysaccharides in feed, facilitating nutrient absorption and utilization. Consequently, it is widely applied in both the brewing and feed industries. Alkaline xylanase (BAX) is generally isolated from microorganisms with an optimal growth pH of 9–11. The assay principle of this kit is as follows: BAX catalyzes the degradation of xylan into reducing oligosaccharides and monosaccharides under alkaline conditions. The resulting reducing sugars undergo a chromogenic reaction with 3,5‑dinitrosalicylic acid (DNS) in a boiling water bath, generating a colored product with a characteristic absorption peak at 540 nm. The color intensity of the reaction solution is directly proportional to the amount of reducing sugars produced by enzymatic hydrolysis. Therefore, the BAX activity can be calculated by measuring the rate of increase in absorbance at 540 nm.