乙酰辅酶A是糖、脂肪、蛋白质三大营养物质代谢的枢纽性中间产物，经三羧酸循环和氧化磷酸化彻底氧化供能，亦是脂肪酸、酮体、胆固醇等合成的前体。本试剂盒利用苹果酸脱氢酶和柠檬酸合酶偶联反应测定其含量：乙酰辅酶A与草酰乙酸在柠檬酸合酶催化下生成柠檬酸，同时偶联的苹果酸脱氢酶反应生成NADH，NADH生成量与乙酰辅酶A含量成正比，通过测定340 nm处吸光度上升量即可定量。
Acetyl‑CoA is a central intermediate in the metabolism of carbohydrates, lipids, and proteins — the three major classes of nutrients. It is completely oxidized to provide energy via the tricarboxylic acid (TCA) cycle and oxidative phosphorylation, and also serves as a precursor for the biosynthesis of fatty acids, ketone bodies, cholesterol, and other compounds. This kit determines acetyl‑CoA content using a coupled reaction system involving malate dehydrogenase and citrate synthase. Acetyl‑CoA reacts with oxaloacetate in the presence of citrate synthase to form citrate. Concurrently, the coupled malate dehydrogenase reaction generates NADH. The amount of NADH produced is directly proportional to the acetyl‑CoA content, allowing for quantitative determination by measuring the increase in absorbance at 340 nm.
危险品化学品经营许可证（带存储）