计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
乙酰辅酶A羧化酶(ACC,EC 6.4.1.2)广泛存在于生物界,催化乙酰辅酶A羧化生成丙二酰辅酶A,是脂肪酸和次生代谢产物合成的关键酶,其活性决定脂肪酸合成速度;本试剂盒采用偶联酶法,ACC催化生成的ADP经丙酮酸激酶和乳酸脱氢酶依次作用使NADH氧化为NAD⁺,通过检测340 nm处NADH下降量即可计算ACC酶活力。
Acetyl‑CoA carboxylase (ACC, EC 6.4.1.2) is widely present in the biological world. It catalyzes the carboxylation of acetyl‑CoA to malonyl‑CoA and is a key enzyme in the biosynthesis of fatty acids and secondary metabolites, with its activity determining the rate of fatty acid synthesis. This kit employs a coupled enzyme method: the ADP generated by the ACC‑catalyzed reaction is sequentially acted upon by pyruvate kinase (PK) and lactate dehydrogenase (LDH), leading to the oxidation of NADH to NAD⁺. The ACC activity can be calculated by measuring the decrease in NADH at 340 nm.