计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
乙酰乳酸合成酶(ALS,EC 2.2.1.6)是支链氨基酸合成的关键酶,仅存在于植物和微生物中,是绿色除草剂的重要靶标。本试剂盒采用显色法测定ALS活力:ALS催化丙酮酸生成乙酰乳酸,经硫酸脱羧为乙酰甲基甲醇,再与显色剂反应生成525 nm处有特征吸收的有色产物,通过检测其生成速率即可计算酶活性。
Acetolactate synthase (ALS, EC 2.2.1.6) is a key enzyme in the biosynthesis of branched‑chain amino acids. It is present exclusively in plants and microorganisms, making it an important target for green herbicides. This kit employs a colorimetric method for the determination of ALS activity. The principle is as follows: ALS catalyzes the formation of acetolactate from pyruvate. The acetolactate is then decarboxylated to acetoin by treatment with sulfuric acid, which subsequently reacts with a chromogenic reagent to produce a colored product with a characteristic absorption peak at 525 nm. ALS activity can be calculated by measuring the rate of formation of this product.