I. Formula (per liter)
Glucose 10.0 g
Peptone 8.0 g
Yeast extract powder 2.0 g
Sodium acetate trihydrate 17.0 g
Sodium chloride 0.002 g
Dipotassium hydrogen phosphate 0.1 g
Potassium dihydrogen phosphate trihydrate 0.1 g
Magnesium sulfate heptahydrate 0.04 g
Ferrous sulfate heptahydrate 0.002 g
Manganese sulfate monohydrate 0.002 g
Agar 12.0 g
Total: 49.2 g
Final pH: 6.8 ± 0.1 (25°C)
II. Preparation Method
1. Weigh 49.2 g of this product (sufficient to prepare 1 liter of medium).
2. Add the weighed material to approximately 800 mL of distilled or deionized water, heat to boiling with constant stirring until completely dissolved.
3. Adjust volume to 1000 mL with distilled or deionized water.
4. Dispense into suitable containers.
5. Autoclave at 121°C for 15 minutes.
6. After sterilization, shake immediately to prevent agar from settling and solidifying at the bottom of the vessel.
7. When cooled to approximately 50–55°C, pour into plates or prepare slants; allow to solidify before use.
III. Usage Instructions
This medium is suitable for the preparation of microbial cultures, used for the preservation, subculture, and stock maintenance of microbial strains.
危险品化学品经营许可证（带存储）