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BioReagent,适用于微生物学 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
发根农杆菌培养基(YEB) 适合发根农杆菌的活化、分离与纯化培养,营养配比均衡,可稳定支持发根农杆菌生长。
培养基配方 (每升):
| 组分 | 含量 |
| 蛋白胨 | 5.0g |
| 牛肉浸粉 | 5.0g |
| 酵母浸粉 | 1.0g |
| 蔗糖 | 5.0g |
| 无水硫酸镁 | 0.02g |
| 琼脂 | 15.0g |
| pH (25℃) | 7.4±0.2 |
使用说明:
称取本品 31.5g 于 1L 蒸馏水中,搅拌加热煮沸至完全溶解,分装,121℃ 高压灭菌 15 分钟,冷至47~50℃,无菌操作添加过滤除菌的抗生素溶液 (如卡那霉素100mg/L) 混匀,倾注平板。
注意事项:
1、操作时避免与皮肤、眼睛直接接触,如不慎接触,请立即用大量清水冲洗。
2、本品仅用于实验室科研实验,不可用于临床诊断。
YEB Medium is suitable for the activation, isolation and purification of Agrobacterium rhizogenes. It has a balanced nutrient composition and can support stable growth of Agrobacterium rhizogenes.
Medium Formula (per liter):
| Component | Content |
| Peptone | 5.0g |
| Beef Extract | 5.0g |
| Yeast Extract | 1.0g |
| Sucrose | 5.0g |
| Magnesium Sulfate | 0.02g |
| Agar | 15.0g |
| pH (25℃) | 7.4±0.2 |
Instructions for Use:
Weigh 31.5 g of this product into 1 L of distilled water, stir and heat to boiling until completely dissolved, dispense, autoclave at 121 °C for 15 minutes, cool to 47~50 °C, add filter‑sterilized antibiotic solution (e.g., kanamycin 100 mg/L) under aseptic conditions, mix well and pour plates.
Precautions:
1. Avoid direct contact with skin and eyes during handling. In case of accidental contact, rinse immediately with plenty of clean water.
2. This product is for laboratory research only and is not intended for clinical diagnosis.
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查看 BioReagent 级别指南 → 查看 Suitable for microbiology 级别指南 →