BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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本染色液为经典Karnovsky-Roots亚铁氰化铜法染色体系，用于组织切片中乙酰胆碱酯酶（AChE）活性的定位检测。 胆碱酯酶（ChE）分为两类：乙酰胆碱酯酶（AChE，真性胆碱酯酶） 主要分布于神经元胞质及神经-肌肉接头（运动终板），负责水解乙酰胆碱，发挥生理调节作用；假性胆碱酯酶（PsChE） 主要存在于血浆、胰腺及唾液腺，生理功能尚不明确。 染色原理：乙酰胆碱酯酶水解底物碘化乙酰硫代胆碱，释放乙酸与硫代胆碱；硫代胆碱的巯基（-SH）将铁氰化钾还原为亚铁氰化钾，后者与铜离子结合，在酶活性位点生成不溶性红棕色至深棕色亚铁氰化铜沉淀，从而精确定位显示酶活性部位。 产品特点与应用：本法操作简便，酯酶扩散现象少；但底物对组织穿透能力较差，适用于冰冻切片中中枢及周围神经纤维形态与酶活性观察。科研层面可用于巨结肠、肠神经元发育异常相关组织学研究；有机磷农药中毒样本中乙酰胆碱酯酶被抑制，酶活性下降表现为阴性染色结果。阳性染色部位呈红棕色至深棕色。 提示：本品含有重金属盐组分，具有刺激性；操作全程请佩戴手套及护目镜，避免皮肤黏膜接触；废液请按有害化学品规范处置。 仅限科研使用，不可用于临床诊断。
This staining solution is based on the classic Karnovsky-Roots ferrocyanide-copper method for the localization of acetylcholinesterase (AChE) activity in tissue sections. Cholinesterases (ChE) are classified into two types: Acetylcholinesterase (AChE, true cholinesterase), which is primarily distributed in neuronal cytoplasm and at neuromuscular junctions (motor endplates), where it hydrolyzes acetylcholine and plays a key role in physiological regulation; and Pseudocholinesterase (PsChE, butyrylcholinesterase), which is mainly found in plasma, pancreas, and salivary glands, though its physiological function remains unclear. Staining Principle: Acetylcholinesterase hydrolyzes the substrate acetylthiocholine iodide, releasing acetic acid and thiocholine. The thiol group (-SH) of thiocholine reduces potassium ferricyanide to potassium ferrocyanide, which then combines with copper ions to form an insoluble reddish-brown to dark brown cupric ferrocyanide precipitate at the enzyme active site, thereby precisely localizing enzyme activity. Features and Applications: This method is simple to perform and exhibits minimal enzyme diffusion artifacts; however, substrate penetration into tissues is relatively limited. It is suitable for observing the morphology and enzyme activity of central and peripheral nerve fibers in frozen sections. At the research level, it can be applied to histological studies of Hirschsprung's disease and intestinal neuronal dysplasia. In samples from organophosphate poisoning, acetylcholinesterase activity is inhibited, resulting in negative staining. Positive staining appears as reddish-brown to dark brown precipitates. Note: This product contains heavy metal salts and is an irritant. Wear gloves and safety goggles throughout the entire procedure, and avoid skin and mucous membrane contact. Dispose of waste in accordance with hazardous chemical regulations. For research use only. Not for clinical diagnosis.
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