铝染色液 (Lillie铝试剂法)

货号: A1529145
有货
级别和纯度: BioReagent ? 生物试剂级(BioReagent)—— 经测试适用于生命科学和分子生物学。适用于需要生物相容性的细胞培养、检测和生化工作。
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规格
库存
价格
数量
3×50ml
A1529145-3×50ml
期货 Stock Image
¥899.90
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为什么选择此级别

BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

2-8°C储存,避光,室温。常规运输,低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

铝在机体大部分经消化道随粪便排出,小部分在睾丸、肾、脾、肌肉、骨骼和脑组织内蓄积。铝在脑中蓄积可引起大脑神经的退化,记忆力衰退,智力和性格也会受到影响,甚至呈现老年性痴呆。当体内铝蓄积量超过正常的 5~16 倍时,可抑制肠道对磷的吸收,干扰体内正常的钙、磷新陈代谢。

铝染色液 (Lillie 铝试剂法) 的原理在于铝试剂与铝离子结合生成红色的络合物,染色过程中应避免使用含汞的固定剂,适用于石蜡切片。该试剂仅用于科研领域,不适用于临床诊断或其他用途。

组分与储存

储运单元货号储运单元名称组分货号组分名称物理外观3×50mL储存温度
A1529145NT铝染色液(Lillie铝试剂法)(常温组分) A1529145A铝试剂粉末1g室温,避光


A1529145B固绿染色液液体50mL室温
A1529145WT铝染色液(Lillie铝试剂法)(2-8℃组分) A1529145CLillie 缓冲液液体50mL2-8℃.


A1529145DLillie 脱色液液体50mL2-8℃.

注:发货时各组分依据储存温度(室温/2-8℃)独立包装,差异化温控运输以保障产品性能。同一订单含多温区物料可能分批次送达,请以实际收货为准。收货后按组分表标注温度分类存放,有效期自生产之日起 12 个月。

自备材料:

1、系列乙醇、蒸馏水、二甲苯或环保浸蜡脱蜡透明液、中性树胶

2、电子天平、水浴锅、微波炉、塑料玻片染色缸

操作步骤 (仅供参考):

1、常规固定,常规浸蜡包埋,切片 5 μm,脱蜡至蒸馏水。

2、配制铝试剂染色液:临用前,按 铝试剂: Lillie 缓冲液 =10 mL :0.2 g 混合,即为铝试剂染色液,即配即用,配制后 2-8℃短期保存。 修改为 铝试剂: Lillie 缓冲液 = 0.2 g:10 mL 混合

3、提前预热铝试剂染色液至 80~85℃,倒入塑料玻片染色缸,载玻片浸入铝试剂染色液并置于微波炉中,设置 2 档 (120 W) 加热 30 s;取出染缸,置于室温 10 min,切片用蒸馏水冲洗 3 次。

4、加入 Lillie 脱色液 5 s,蒸馏水冲洗 3 次。

5、固绿染色液复染 3 min,蒸馏水冲洗 3 次。

6、立片、风干。

7、稍微浸入二甲苯或脱蜡透明液透明,中性树胶封固。

染色结果:

铝红色
背景绿色

注意事项:

1、微波炉加热时应注意微波炉的功率,功率过大时,应减少加热时间。

2、为了您的安全和健康,请穿实验服并戴一次性手套操作。

3、试剂开封后请尽快使用,以防影响后续实验效果。

 

Most aluminum in the body is excreted via the digestive tract in feces, while a small portion accumulates in the testes, kidneys, spleen, muscles, bones and brain tissue. Accumulation of aluminum in the brain can lead to degeneration of cerebral nerves, memory loss, impaired intelligence and personality changes, and may even cause symptoms resembling senile dementia. When the bodily aluminum level exceeds the normal value by 5‑16‑fold, it can inhibit intestinal phosphorus absorption and disturb normal calcium‑phosphorus metabolism in vivo.

Aluminum Stain Solution (Lillie Aluminon Method) is that aluminon binds to aluminum ions to form red complexes. Mercury‑containing fixatives should be avoided during staining. This kit is suitable for paraffin sections. For research use only; not intended for clinical diagnosis or other clinical applications.

Components & Storage

Storage and Transport Unit Item No.Storage and Transport Unit NameComponent No.Component NameAppearance3×50mLStorage
A1529145NTAluminum Stain Solution (Lillie Aluminon Method)(RT Components)A1529145AAluminonPowder1gRoom temperature. Store in the dark


A1529145BFast Green Stain SolutionLiquid50mLRoom temperature
A1529145WTAluminum Stain Solution (Lillie Aluminon Method)(2-8℃ Components)A1529145CLillie BufferLiquid50mL2-8℃.


A1529145DLillie Decolorizing SolutionLiquid50mL2-8℃.

Note: 
Each component is individually packaged according to its storage temperature (Room temperature/2-8 °C) upon shipment, with differentiated temperature‑controlled transportation to guarantee product performance. Orders containing materials requiring multiple temperature zones may be delivered in separate batches; please refer to the actual received goods. Upon receipt, store components separately at the temperatures specified in the component list. The shelf‑life is 12 months from the date of manufacture.

Required Materials (not supplied):

1. Graded ethanol, distilled water, xylene or environmentally‑friendly paraffin‑embedding & dewaxing clearing solution, neutral balsam

2. Electronic balance, water bath, microwave oven, plastic slide staining jar

Procedures (for reference only):

 1. Perform routine fixation and paraffin‑embedding. Prepare 5 μm‑thick sections and dewax to distilled water.

2. Preparation of aluminon working staining solution: Mix  Aluminon and  Lillie Buffer at a ratio of 10 mL : 0.2 g immediately before use. Use the mixed solution right after preparation; short‑term storage is allowed at 2-8 °C.    修改为 Mix  Aluminon and  Lillie Buffer at a ratio of 0.2 g:10 mL immediately before use. 

3. Pre‑warm the aluminon working staining solution to 80‑85 °C and pour it into a plastic slide‑staining jar. Immerse slides in the staining solution and place the jar in a microwave oven. Heat at Level 2 (120 W) for 30 s. Remove the staining jar, hold at room temperature for 10 min, then rinse sections three times with distilled water.

4. Immerse slides in Lillie Decolorizing Solution for 5 s, then rinse three times with distilled water.

5. Counter‑stain with Fast Green Stain Solution for 3 min, then rinse three times with distilled water.

6. Place slides vertically and allow air‑drying.

7. Clear slides briefly in xylene or dewaxing‑clearing solution, and mount with neutral balsam.

Staining Results:

TargetColor
AluminumRed
BackgroundGreen

Precautions:

1. Pay attention to microwave power during heating; reduce heating time if power is too high.

2. Wear a lab coat and disposable gloves for personal safety and health during operation.

3. Use the reagent promptly after opening to avoid compromised experimental performance.

规格

规格或纯度
BioReagent
英文名称
Aluminum Stain Solution (Lillie Aluminon Method)
应用
Staining
样品类型
组织
敏感性
Light-sensitive
储存与运输
储存条件
2-8°C储存,避光,室温
运输条件
常规运输,低温运输
稳定性与储存
各组分在相应的储存条件下保质期12个月。
组分与储存
储运单元货号储运单元名称组分货号
组分名称
物理外观3×50mL储存温度
A1529145NT铝染色液(Lillie铝试剂法)(常温组分) A1529145A铝试剂粉末1g室温,避光


A1529145B
固绿染色液
液体50mL室温
A1529145WT铝染色液(Lillie铝试剂法)(2-8℃组分)A1529145CLillie 缓冲液液体50mL2-8℃.


A1529145D
Lillie 脱色液

液体50mL2-8℃.
注:发货时各组分依据储存温度(室温/2-8℃)独立包装,差异化温控运输以保障产品性能。同一订单含多温区物料可能分批次送达,请以实际收货为准。收货后按组分表标注温度分类存放,有效期自生产之日起 12 个月。

技术文档

📋 安全数据表 (SDS)

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技术文档和文章
溶液计算器

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