固定的目的在于保存细胞和组织的原有形态结构，固定剂能阻止内源性溶酶体酶对自身组织和细胞的自溶、抑制细菌和霉菌的生长，固定剂通过凝固、生成添加化合物等使蛋白质内部结构发生改变，从而使酶失活。固定液分为醛类固定液、汞类固定液、醇类固定液、氧化剂类固定液、苦味酸盐类固定液等，较为常用的是醛类中的福尔马林、醇类中的乙醇。
酒精-福尔马林-甘油固定液主要由乙醇、福尔马林、甘油组成，该固定液属于脱水-交联固定剂，在辨认特定抗体的免疫组化反应中能产生极好的结果，尤其适用于长期保存材料。
该试剂仅用于科研领域，不适用于临床诊断或其他用途。
操作步骤（仅供参考）：
1、按实验具体要求操作。
2、一般标本固定时间控制1～4 h/mm，大多数控制在4～24 h，大标本应适当延长固定时间。
注意事项：
1、酒精-福尔马林-甘油固定液有一定刺激性和腐蚀性，请在通风环境下小心操作。
2、组织取材的厚度不同，固定时间也不同；常规活检组织比较适合的厚度为2～4 mm，一般不超过6 mm，对组织恰当的选材有利于固定液的渗透。
3、固定液的容量应足够，一般固定液与组织块的体积比率应大于10：1；如果容积不够大，可以在固定期间更换1～3次固定液。
4、温度对固定的影响很明显，提高温度可以加速固定作用，但温度不宜过高。
5、取出新鲜组织后应及时固定，无法及时固定时应保存于生理盐水中及时送检。
6、为了您的安全和健康，请穿实验服并戴一次性手套操作。
7、试剂开封后请尽快使用，以防影响后续实验效果。
The purpose of fixation is to preserve the original morphological structure of cells and tissues. Fixatives can prevent autolysis of tissues and cells by endogenous lysosomal enzymes and inhibit the growth of bacteria and fungi. Fixatives alter the internal structure of proteins through coagulation, formation of addition compounds, etc., thereby inactivating enzymes. Fixatives are divided into aldehyde fixatives, mercury-based fixatives, alcohol-based fixatives, oxidizing agent-based fixatives, picrate-based fixatives, etc. The most commonly used are formalin among aldehydes and ethanol among alcohols.
Alcohol‑Formalin‑Glycerol Fixative is mainly composed of ethanol, formalin, and glycerol. This fixative belongs to dehydrating-crosslinking fixatives and can produce excellent results in immunohistochemical reactions for identifying specific antibodies, and is especially suitable for long-term preservation of materials.
This reagent is for research use only and is not suitable for clinical diagnosis or other purposes.
Procedure (for reference only):
1.Operate according to the specific requirements of the experiment.
2.Generally, the fixation time for specimens is controlled at 1–4 h/mm, most are controlled at 4–24 h, and the fixation time for large specimens should be appropriately extended.
Precautions:
1.Alcohol-Formalin-Glycerol Fixative has certain irritancy and corrosiveness. Please operate carefully in a well-ventilated environment.
2.The fixation time varies with the thickness of the tissue sample. The suitable thickness for routine biopsy tissues is generally 2–4 mm, and generally should not exceed 6 mm. Proper selection of tissue is beneficial for the penetration of the fixative.
3.The volume of the fixative should be sufficient. Generally, the volume ratio of fixative to tissue block should be greater than 10:1. If the volume is insufficient, the fixative can be replaced 1–3 times during fixation.
4.Temperature has a significant effect on fixation. Increasing the temperature can accelerate fixation, but the temperature should not be too high.
5.Fresh tissue should be fixed promptly after removal. If it cannot be fixed promptly, it should be stored in normal saline and submitted for examination in a timely manner.
6.For your safety and health, please wear a laboratory coat and disposable gloves during operation.
7.After opening the reagent, please use it as soon as possible to prevent affecting subsequent experimental results.
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