安德烈指示剂，又称安德烈氏指示剂、Andrade 指示剂（也写作 Andzed 指示剂），由酸性品红、碱等组分配制而成，为浅黄至淡稻草色液体。酸性环境下呈粉红色或玫瑰红色，碱性环境下呈黄色或浅黄色。变色范围：pH≈5.0（粉红）～8.0（黄）；实际判读常用参考：pH 5.5 附近显红色，pH 7.5 附近显黄色。

主要应用场景：

① 细菌糖发酵试验：用于葡萄糖、乳糖、蔗糖、甘露醇等糖类或糖醇发酵产酸判定（如 Andrade 蛋白胨水、BAM M13 糖类肉汤体系）。

② 尿道菌分离培养基：在含 Andrade 指示剂的 CLED 琼脂上，区分乳糖发酵菌（呈粉红色）与非发酵菌（保持黄绿色）。

③ 教学及食品微生物检验：配套 SN/T 0168 等标准中的糖发酵管实验。

本品主要用于微生物碳水化合物代谢实验；可分解糖、醇、苷类并产生酸的细菌，会使培养基变为红色。

本试剂仅用于科研领域，不适用于临床诊断或其他用途。

操作步骤（仅供参考）：

1、使用前需确认本品为浅黄至淡稻草色液体，如为红色可加适当量的氢氧化钠并放置24 h至颜色符合要求即可使用。

2、基础蛋白胨水 (pH 7.2±0.2)灭菌冷却后，按1 L基础液加10 mL指示剂 (即1%)。

3、糖类单独配成5%-10%无菌滤液，按0.5%-1%终浓度加入，分装小管 (Durham管倒管查产气)。

4、35-37℃培养18-24 h，观察颜色和性状的变化：变红色或粉红色，表示产酸；不变黄红，表示不发酵；倒管有气泡，说明产气。

注意事项：

1、本品未经无菌处理，如需严格无菌，请在无菌条件下过滤后使用。

2、必须充分脱色：以浅黄透明为宜，如为紫红色或红色，会导致背景太红、终点看不清。

3、基础培养基pH必须调至 7.2 ± 0.2，灭菌后于 50 - 60℃时在无菌条件下加入指示剂。

4、培养别超时：CLED类平板培养>24 h可能被优势发酵菌整体变粉，掩盖非发酵菌落。

5、不与BTB混用导致判色冲突：部分 CLED 同时含溴百里酚蓝 +Andrade，以局部菌落周围粉红晕为发酵判据，勿只看全局。

6、试剂开封后请尽快使用，以防影响后续实验效果。

7、为了您的安全和健康，请穿实验服并戴一次性手套操作。

Andrade’s Indicator, also known as Andzed Indicator, is composed of acid fuchsin and alkali. It appears pale yellow to pale straw-colored liquid. It turns pink/rose-red under acidic conditions and yellow/light yellow under alkaline conditions. Its pH transition range is approximately pH 5.0 (pink) to pH 8.0 (yellow). In routine interpretation, it is red near pH 5.5 and yellow near pH 7.5.

Main applications:

① Bacterial carbohydrate fermentation test: for detection of acid production from glucose, lactose, sucrose, mannitol and other sugars or sugar alcohols (e.g., Andrade Peptone Water, BAM M13 carbohydrate broths).

② Medium for urinary bacterial isolation: used in CLED Agar with Andrade's Indicator to differentiate lactose fermenters (pink) from non-fermenters (yellow-green).

③ Teaching and food microbiological testing: used in carbohydrate fermentation tubes specified in standards such as SN/T 0168.

This product is used for microbial carbohydrate metabolism assays. Bacteria capable of fermenting sugars, alcohols and glycosides to produce acid will turn the medium red.

For research use only. Not for clinical diagnosis or other applications.

Procedure (for reference only):

1.Before use, confirm that this product is a light yellow to pale straw-colored liquid. If it is red, add an appropriate amount of sodium hydroxide and let it stand for 24 h until the color meets the requirements before use.

2.After basal peptone water (pH 7.2 ± 0.2) is sterilized and cooled, add 10 mL of indicator per 1 L of basal medium (i.e., 1%).

3.Prepare sugars separately as 5%–10% sterile filtrates, add at a final concentration of 0.5%–1%, and dispense into small tubes (use Durham tubes to check gas production).

4.Incubate at 35–37 ℃ for 18–24 h and observe changes in color and characteristics: turning red or pink indicates acid production; no change to yellow-red indicates no fermentation; bubbles in the inverted tube indicate gas production.

Precautions:

1.This product has not been sterilized. If strict sterility is required, please filter it under aseptic conditions before use.

2.Sufficient decolorization is required: light yellow and transparent is preferable. If it is purple-red or red, the background will be too red and the endpoint will be difficult to distinguish.

3.The pH of the basal medium must be adjusted to 7.2 ± 0.2. After sterilization, add the indicator under aseptic conditions at 50–60 ℃.

4.Do not over-incubate: CLED plates cultured for >24 h may be turned pink overall by dominant fermenting bacteria, masking non-fermenting colonies.

5.Do not mix with BTB, as this may cause color interpretation conflicts: some CLED media contain both bromothymol blue + Andrade. Use the pink halo around local colonies as the criterion for fermentation, and do not judge only by the overall color.

6.After opening the reagent, please use it as soon as possible to prevent affecting subsequent experimental results.

7.For your safety and health, please wear a laboratory coat and disposable gloves during operation.

规格

别名

安德拉德指示剂 | Andrade 氏指示剂 | Andzed指示剂 | 安德烈氏指示剂

英文别名

Andrade's acid fuchsin indicator

规格或纯度

BioReagent, 适用于微生物学, pH ≈ 5.0 (粉红) - 8.0 (黄)

稳定性与储存

Store at RT long term (12 months).

英文名称

Andrade’s Indicator

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室温

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常规运输

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生物试剂/缓冲液

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安德烈指示剂

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