妇科标本使用专用采集器按常规方法采集后，将采集器放入装有保存液的样品瓶内充分刷洗；建议上下、左右多方向刷洗，不可单向搅动，防止含细胞的黏液附着于采集器表面。多数妇科标本无需额外处理，采集后可直接置于保存液小瓶中。约 15% 标本因含大量血液或黏液，会干扰阅片判读，需进行标本均质化前处理。
常用标本前处理方法包含乙酸消化法、乙酸‑二硫苏糖醇 (DTT) 消化法等。乙酸消化液 (清洗液) 主要由冰乙酸、纯化水配制而成，适用于妇科标本及非妇科标本（穿刺液、尿液、痰液、胸腔积液、渗出液，呼吸道、胃肠道、乳腺等分泌物）的均质化前处理，尤其适合血液、黏液含量偏高的标本。 本试剂仅用于科研领域，不适用于临床诊断或其他用途。
操作步骤 (仅供参考)：
(一) 黏液标本 (如痰液)
1、取样与加液：取适量痰液标本 (黄豆粒大小) 或其他样本放入含有 20ml 生理盐水的离心管中，并加入适量 (约 10ml) 的乙酸消化液 (清洗液)；如标本含血较多，可直接加 20～30ml 本消化液。
2、振荡离心：摇晃混匀 10min，1500～2000r/min 离心 5～10min，弃上清；如肉眼可见细胞层仍有血或黏液则重复上述步骤。
3、转移和制片：弃去上清后将沉淀倒入盛有细胞保存液的标本瓶中，静置≥15min，制片。
(二) 穿刺液标本
1、取样与加液：标本放入离心管，加入 30ml 清洗液，血性标本加入本品 20～30ml。
2、振荡离心：1500～2000r/min 振荡 10min，1500～2000r/min 离心 5～10min，弃上清；肉眼仍可见血或黏液，重复步骤。
3、转移和制片：沉淀转入细胞保存液瓶，静置≥15min，制片。
(三) 支气管镜刷洗液
1、刷洗：将支气管镜刷放入 30ml 清洗液中刷洗。若标本含血较多，可加本品 20～30ml。
2、振荡离心：1500～2000r/min 振荡 10min，1500～2000r/min 离心 5～10min，弃上清；肉眼仍可见血或黏液，重复步骤。
3、转移和制片：沉淀转入细胞保存液瓶，静置≥15min，制片。
注意事项：
1、本产品含有冰乙酸，具有腐蚀性和刺激性气味。操作时请在通风良好的环境中进行。
2、样本置入保存液中以微透明为佳，如果浑浊，应重新稀释。
3、消化液的加入量应根据标本含血量灵活调整。过量使用可能导致细胞形态受损，过少则去血效果不佳。建议初次使用时参照标准量 (20~30ml)，后续根据经验微调。
4、对于严重污染的标本，不要强行一次性处理干净，应通过 “重复离心 - 加液” 的方式逐步去除杂质，以免破坏有效细胞成分。
5、溶液如出现浑浊、沉淀请勿继续使用。
6、为了您的安全和健康，请穿实验服并戴一次性手套操作。
7、试剂开封后请尽快使用，以防影响后续实验效果。
Gynecological specimens are collected with dedicated samplers following standard procedures. Place the sampler into a vial with preservative solution and thoroughly rinse it by moving up, down, left and right. Unidirectional stirring should be avoided to prevent cell-containing mucus from adhering to the sampler surface. Most gynecological specimens require no additional treatment and can be directly transferred into preservative vials. Approximately 15% of specimens contain excessive blood or mucus, which interferes with slide evaluation and requires homogenization pre-treatment.
Common specimen pre-treatment protocols include acetic acid digestion and acetic acid‑dithiothreitol (DTT) digestion. Acetic Acid Digestion Solution (Wash Solution) is composed of glacial acetic acid and purified water. It is used for homogenization pre-treatment of gynecological and non-gynecological specimens (aspirates, urine, sputum, pleural effusion, exudates, secretions from respiratory tract, gastrointestinal tract, breast, etc.), especially for specimens with high levels of blood or mucus.
For research use only. Not for clinical diagnosis or other applications.
Operating Procedures (For Reference Only):
(I) Mucus Specimens (e.g. Sputum)
1. Sampling and reagent addition: Transfer an appropriate amount of sputum specimen (soybean grain size) or other sample into a centrifuge tube containing 20 mL normal saline. Add approximately 10 mL Acetic Acid Digestion Solution (Wash Solution). For heavily blood-contaminated specimens, add 20–30 mL of this solution directly.
2. Vortex and centrifugation: Vortex and mix for 10 min, then centrifuge at 1500–2000 r/min for 5–10 min. Discard the supernatant. Repeat the procedure if blood or mucus is still visible in the cell pellet.
3. Transfer and slide preparation: Discard the supernatant and transfer the pellet into a specimen vial with cell preservative solution. Let stand for ≥15 min before slide preparation.
(II) Aspirate Specimens
1. Sampling and reagent addition: Place the specimen into a centrifuge tube and add 30 mL wash solution. For bloody specimens, add 20–30 mL of this product.
2. Vortex and centrifugation: Vortex at 1500–2000 r/min for 10 min, then centrifuge at 1500–2000 r/min for 5–10 min. Discard the supernatant. Repeat if blood or mucus remains visible.
3. Transfer and slide preparation: Transfer the pellet into the cell preservative vial. Let stand for ≥15 min before slide preparation.
(III) Bronchoscopic Brush Washings
1. Brushing: Immerse the bronchoscopic brush into 30 mL wash solution and rinse. Add an additional 20–30 mL of this product for blood-rich specimens.
2. Vortex and centrifugation: Vortex at 1500–2000 r/min for 10 min, then centrifuge at 1500–2000 r/min for 5–10 min. Discard the supernatant. Repeat if blood or mucus remains visible.
3. Transfer and slide preparation: Transfer the pellet into the cell preservative vial. Let stand for ≥15 min before slide preparation.
Precautions:
1. This product contains glacial acetic acid, which is corrosive and emits an irritating odor. Perform operations in a well-ventilated area.
2. Specimens in preservative solution should appear slightly translucent. Re-dilute if turbidity occurs.
3. Adjust the volume of digestion solution according to the blood content of specimens. Excessive dosage may damage cell morphology; insufficient dosage results in poor blood removal. Use the standard volume (20–30 mL) for initial trials and fine-tune based on experience.
4. Do not attempt complete one-step cleanup for heavily contaminated specimens. Remove impurities gradually via repeated centrifugation and reagent addition to preserve intact target cellular components.
5. Discontinue use if the solution becomes turbid or forms precipitates.
6. Wear a lab coat and disposable gloves during operation for personal safety and health protection.
7. Use the reagent promptly after opening to avoid compromising experimental results.
危险品化学品经营许可证（带存储）