计算溶液所需的质量、体积或浓度。
试剂级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
人工海水又称人造海水,英文名称为 Artificial Sea Water,用于替代天然海水,方便实验室条件下培养真菌等试验的需求。
人工海水(ASW,Mocledon)参照 McEwen (1917)氯度19.00‰模拟海水组成,经中文文献以“Mocledon 人工海水”转引(Shi et al., 2006;《地学辞典》),盐度33.4‰,Mocledon ASW 是中文水产/植物耐盐圈子里的惯用叫法。
人工海水粉剂 (ASW, Mocledon)通过精确模拟天然海水的离子组成,消除了天然海水来源的变异性,为各类实验提供了高度可重复的标准环境。产品为干粉状,具有保存方便、配制简单、批次稳定性好的特点。其用途如下:
①微生物学:海洋真菌、放线菌及其他嗜盐微生物的分离、培养与保藏。
②海洋生物学:海洋藻类、无脊椎动物(如海胆、海星、水母)卵或幼体的培养,以及小型海洋生物的暂养。
③分析化学:作为标准基质,用于海水成分分析方法的校正、校准(如盐度、电导率、特定离子电极),以及海洋腐蚀相关试验。
本产品主要由氯化钠、氯化镁、硫酸镁、氯化钙、氯化钾、硼酸、碳酸氢钠、溴化钠等组成,总盐度约为33.4‰,pH约为7.8~8.2。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
组分与储存
注:组分 A1539078A 装量偏差:33.43 g ±0.25 g (1 L);334.3 g ±2.5 g (10 L);组分 A1539078B 装量偏差:1.175 g ±0.025 g (1 L);11.75 g ±0.25 g (10 L)。 |
操作步骤(仅供参考,1L规格):
1、将试剂一溶解于900ml去离子水中,待完全溶解后,再加入试剂二,充分混合溶解,定容至1升,用稀盐酸或氢氧化钠调整pH值为8.0即可;如有必要,可过滤后使用。
2、如需灭菌,可于121℃高压灭菌15分钟(注意:长时间高温可能导致微量沉淀,通常不影响使用)。
注意事项:
1、试剂一含镁盐、硫酸盐、碳酸盐,溶于水后呈弱碱性,若有浑浊状沉淀,可先用稀盐酸调节pH值至澄清(7.5~8.0)才可进行后续操作。
2、试剂二含钙盐,为白色粉末或球状颗粒,有吸水性,应避免存放于湿度较高的地方。
3、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Artificial seawater (ASW), also known as synthetic seawater, is used as a substitute for natural seawater to facilitate laboratory experiments, such as fungal cultivation.
Artificial Seawater (ASW, Mocledon) is formulated based on McEwen's (1917) simulated seawater composition with a chlorinity of 19.00‰. It has been cited in Chinese literature as "Mocledon Artificial Seawater" (Shi et al., 2006; Dictionary of Earth Sciences), with a salinity of 33.4‰. The term "Mocledon ASW" is commonly used in Chinese aquaculture and plant salt-tolerance research.
Artificial Seawater Powder (ASW, Mocledon) accurately simulates the ionic composition of natural seawater, eliminating variations associated with natural seawater sources and providing a highly reproducible, standardized environment for various experiments. The product is supplied as a dry powder, offering convenient storage, easy preparation, and excellent batch-to-batch consistency. Its applications include:
① Microbiology: Isolation, cultivation, and preservation of marine fungi, actinomycetes, and other halophilic microorganisms.
② Marine Biology: Cultivation of marine algae, eggs or larvae of marine invertebrates (such as sea urchins, starfish, and jellyfish), and temporary maintenance of small marine organisms.
③ Analytical Chemistry: Used as a standard matrix for the calibration and standardization of seawater composition analysis methods (such as salinity, conductivity, and ion-selective electrode measurements), as well as for marine corrosion testing.
This product primarily consists of sodium chloride, magnesium chloride, magnesium sulfate, calcium chloride, potassium chloride, boric acid, sodium bicarbonate, sodium bromide, and other components. It has a total salinity of approximately 33.4‰ and a pH of approximately 7.8–8.2. This reagent is intended for research use only and is not suitable for clinical diagnosis or other purposes.
Contents & Storage
Note: Component A1539078A weight tolerance: 33.43 g ±0.25 g (1 L); 334.3 g ±2.5 g (10 L). Component A1539078B weight tolerance: 1.175 g ±0.025 g (1 L); 11.75 g ±0.25 g (10 L). |
Preparation Procedure (For Reference Only, 1 L Specification):
1. Dissolve Reagent 1 in 900 mL of deionized water. Once completely dissolved, add Reagent 2 and mix thoroughly until fully dissolved. Adjust the final volume to 1 L, then adjust the pH to 8.0 using dilute hydrochloric acid or sodium hydroxide. Filter before use if necessary.
2. If sterilization is required, autoclave at 121 °C for 15 minutes (Note: Prolonged exposure to high temperatures may cause slight precipitation, which generally does not affect its use).
Precautions:
1. Reagent 1 contains magnesium salts, sulfates, and carbonates, and produces a slightly alkaline solution when dissolved in water. If turbidity or precipitation occurs, adjust the pH to 7.5–8.0 using dilute hydrochloric acid until the solution becomes clear before proceeding with subsequent steps.
2. Reagent 2 contains calcium salts and is supplied as a white powder or spherical granules. It is hygroscopic and should not be stored in environments with high humidity.
3. For your safety and health, wear a laboratory coat and disposable gloves when handling this product.
组分货号 | 组分名称 | 物理外观 | 1 L | 10 L | 储存 |
A1539078A | 试剂一 | 固体 | 33.43 g | 334.3 g | 室温 |
A1539078B | 试剂二 | 固体 | 1.175 g | 11.75 g | 室温 |