计算溶液所需的质量、体积或浓度。
概述
Features
Ultrapure quality
Sugar specificity: fucose and terminal fucose residues on complex oligosaccharides and glycoconjugates
Binding affinity for fucose in all binding positions (α1-2, α1-3, α1-4 and α1-6)
Higher affinity towards fucosylated oligosaccharides than native AaL
Not blood group specific
Product description
Recombinant Aleuria aurantia lectin is produced in E.coli and has an amino acid sequence identical to native Aleuria aurantia lectin. AAL is a dimeric lectin with two identical subunits of approximately 36 kDa. Each subunit has five carbohydrate-binding sites (1). The lectin recognizes and binds specifically to fucose and terminal fucose residues on complex oligo saccharides and glycoconjugates. rAAL has binding affinity for fucose in all binding positions (α1-2, α1-3, α1-4 and α1-6) and in contrast to AAL purified from natural sources, rAAL is not contaminated with free fucose yielding higher affinity towards fucosylated oligosaccharides than native AAL (2).
Recombinant AAL hemagglutinates erythrocytes irrespective of blood type (A, B and 0) at the same titers as AAL isolated from natural sources.
AAL has been widely used for analysis and preparation of oligosaccharides and glycoproteins (3). Diagnostic applications include analysis of disease-associated glycosylation on plasma proteins (4). Furhtermore, rAAL can be immobilized and used for affinity chromatography (5).
Applications
Studies of glycoproteins and glycolipids
Purification of membrane proteins
Affinity chromatography
Agglutination studies
Directions for use
The lectin may be reconstituted with 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08 % sodium azide, 0.1 mM Ca++, 0.01 mM Mn++. Spin the vial gently until full dissolution. Aggregation is thought to occur in the presence of high concentrations of 2-mercaptoethanol.
Shipping and storage
The product is shipped at -20°C however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than three years from production date when stored below -20°C. After reconstitution with HEPES buffer, the solution may be stored frozen in working aliquots for up to 12 months.
规格
英文名称
Aleuria aurantia lectin (AAL)
储存条件
-20°C储存
运输条件
超低温运输
名称和识别符
分子类型
蛋白质
高级数据
安全和危险性（GHS）
象形图
信号词
警告
危险声明
H317: 可能引起皮肤过敏反应
H319: 引起严重眼睛刺激
H334: 吸入可能引起过敏或哮喘病症状或呼吸困难
预防措施声明
P261: 避免吸入灰尘/烟雾/气体/雾/蒸汽/喷雾
P280: 戴防护手套/穿防护服/戴防护眼罩/戴防护面具。
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 C2321426 |
分析证书 | A419041 |
技术文档和文章
溶液计算器
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