计算溶液所需的质量、体积或浓度。
消泡剂 SE-15
有货
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
产品描述
消泡剂 SE-15 是一种硅树脂产品，用于防止或减少微生物发酵系统和哺乳动物悬浮培养物中的泡沫。泡沫控制 使细胞损伤最小化，导致蛋白/抗体生成增加。
消泡剂作为两种基本类型的组合物或两者的混合物提供。有机消泡剂是合成的。硅基消泡剂通常被认为是硅氧烷聚合物，也是合成的。我们的供应商通常认为确切的成分是专有的。产品被列为分子生物学等级，并已测试用于细菌发酵。当大肠杆菌或枯草杆菌在添加有效浓度消泡剂的极好肉汤中生长时，未发现明显的抑制作用。这些产品有单独的数据表，可根据要求提供。
组分
消泡剂 SE-15 是一种含有 10% 活性硅和非离子型乳化剂的水性乳液。
制备说明
消泡剂 SE-15 可用 3-10 份冷水预稀释，以辅助分散。预稀释混悬液应立即使用。高压灭菌。 多次高压灭菌不会显着影响性能。根据需要泵入发酵罐。
程序
消泡剂应在发生泡沫之前添加。或者，消泡剂可以按原样使用，方法是在填充喷嘴、处理桶的边缘或悬挂在起泡系统上方的筛网上进行擦拭。消泡剂最常见的应用之一是微生物发酵。由于无法预测消泡剂对特定应用的有效性，因此还应对消泡剂进行测试，以确保在每种微生物的代表性培养条件下具有足够的消泡效果。应使用为最终实验或发酵条件预期的培养基成分、温度、pH、混合和通气等变量。如果消泡剂在这些测试条件下无效，则可以添加更高量的消泡剂或测试不同类型的消泡剂。
应用
消泡剂 SE-15 已用于分枝滚环扩增 (RAM) 扩增缓冲液的制备。它还被用于寻找其对细胞活力、病毒感染性和 PCR（聚合酶链反应）性能的影响。
消泡有机硅在微生物发酵技术中的应用。泡沫层的形成和随后的细胞损伤/损失被发现是影响细胞生长和单克隆抗体 (MAb) 生产的主要问题。补充和无血清培养基。培养基中的表面活性剂通过改变气泡的性质和形成的泡沫的质量对细胞生长产生了深远的影响。
向气泡柱中的杂交瘤细胞悬浮液中添加高浓度有机硅消泡剂可降低使用不含保护成分的培养基时的死亡率。在含有有机硅聚醚的非离子 Tritonä X-100 表面活性剂溶液中的消泡机制，即所谓的“浊点消泡剂”进行了调查。浊点 (CP) 研究表明，由于混合胶束的形成，Triton X-100/有机硅聚醚混合体系的 CP 介于起泡的 CP 和消泡表面活性剂之间。超临界二氧化碳用于直接萃取在分析之前从血浆中提取药物。超临界流体直接通过掺有中性（黄酮）或酸性（酮咯酸）药物的血浆样品。需要在提取之前向血浆中添加消泡剂以避免由于超临界流体使血浆蛋白变性而引起的限流器堵塞。
Antifoam SE-15 is a silicone-based product used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.
Antifoams are supplied as two basic types of composition or mixtures of the two. Organic antifoams are of synthetic origin. Silicone-based antifoams are generally considered to be siloxane polymers and are also synthetic. Our suppliers generally consider the exact composition to be proprietary.Products are listed as Molecular Biology Grade and have been tested for use in bacterial fermentation. No significant inhibition is found when E. coli or B. subtilis is grown in Terrific Broth supplemented with effective concentrations of antifoams. These products have individual data sheets available upon request.
Components
Antifoam SE-15 is an aqueous emulsion containing 10% active silicon and non-ionic emulsifiers .
Preparation Note
Antifoam SE-15 can be pre-diluted with 3-10 parts of cool water to aid in dispersion. Pre-diluted suspensions should be used immediately.Sterilize by autoclaving. Multiple autoclaving will not significantly affect performance. The flow properties of Antifoam SE-15 allow pumping to a fermenter on an asneeded basis.
Procedure
Antifoams should be added prior to the point where foaming occurs. Alternatively, antifoam can be used as supplied, by wiping on filling nozzles, rims of processing vats, or on a screen suspended above the foaming system. One of the most common applications for antifoams is in microbiological fermentation.Since one cannot predict the effectiveness of an antifoam for a particular application, antifoams should also be tested to ensure adequate defoaming effectiveness under representative culture conditions for each microorganism. The variables of medium composition, temperature, pH, mixing, and aeration anticipated for the final experimental or fermentation conditions should be used. If the antifoam is not effective under these test conditions either a higher amount of antifoam can be added or a different type of antifoam can be tested.
Applications
Antifoam SE-15 has been used in the preparation of ramified rolling circle amplification (RAM) amplification buffer. It has also been used to find its effects on cell viability, virus infectivity and PCR (polymerase chain reaction) performance.
The application of antifoam silicones in the microbiological fermentation technique.Foam formation and the subsequent cell damage/ losses in the foam layer were found to be the major problems affecting cell growth and monoclonal antibody (MAb) production in stirred and sparged bioreactors for both serum-supplemented and serum-free media. Surfactants in the culture media had a profound effect on cell growth by changing both the properties of bubbles and the qualities of foam formed.
Addition of high concentrations of silicone antifoam to a suspension of hybridoma cells in a bubble column reduces the death rate when using medium without a protective component.The mechanism of antifoaming in a nonionic Tritonä X-100 surfactant solution with silicone polyethers, the so-called "cloud point antifoams," was investigated. The cloud point (CP) studies showed that the CP of a Triton X-100/silicone polyether mixed system is between the CP of the foaming and the antifoam surfactants, due to mixed micelle formation.Supercritical carbon dioxide was used for the direct extraction of drugs from plasma prior to analysis. The supercritical fluid was directly passed through plasma samples spiked with either a neutral (flavone) or an acidic (ketorolac) drug. The addition of an antifoam agent to the plasma prior to extraction was required to avoid restrictor plugging caused by denaturation of theplasma proteins by the supercritical fluid.
规格
规格或纯度
aqueous emulsion for bacterial and mammalian systems
英文名称
Antifoam SE-15
储存条件
室温
运输条件
常规运输
名称和识别符
分子类型
未知
技术文档
高级数据
化学和物理性质
溶解性
H2O: soluble (effective hot or cold.)
密度
1.0 g/mL at 25 °C
安全和危险性（GHS）
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 E2422382 |
分析证书 | A489557 |
| C2603305 |
分析证书 | A489557 |
| C2603307 |
分析证书 | A489557 |
| J2319032 |
分析证书 | A489557 |
| I2512111 |
分析证书 | A489557 |
| I2512110 |
分析证书 | A489557 |
| C2520219 |
分析证书 | A489557 |
| E2327854 |
分析证书 | A489557 |
| E2422383 |
分析证书 | A489557 |
| C2522091 |
分析证书 | A489557 |
| C2429059 |
分析证书 | A489557 |
| J2319031 |
分析证书 | A489557 |
| E2327855 |
分析证书 | A489557 |
| E2327858 |
分析证书 | A489557 |
| E2327853 |
分析证书 | A489557 |
| B2322559 |
分析证书 | A489557 |
| B2322580 |
分析证书 | A489557 |
溶液计算器
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