计算溶液所需的质量、体积或浓度。
Alpha Galactosidase
有货
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Product Description
Alpha Galactosidase from E. coli cleaves α(1-3)- and α(1-6)-linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins. There is no activity on α(1-4) linked galactose. It is particularly efficient for removing α-linked galactose under conditions where the pH must be neutral or above, for example, with living cells.
Molecular Weight
~80,000 daltons
Contents
Alpha galactosidase in 50 mM sodium phosphate, pH 7.5
included with 20 µL and 60 µl pack sizes:
Reaction buffer - 250mM Sodium phosphate, pH 6.5
Specificity
Non-reducing terminal alpha-(1-3)- and alpha-(1-6)- galactose. There is no activity on alpha-(1-4)-galactose.
Stability
Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity.
Specific Activity
One unit of alpha-(1-3,6) Galactosidase is defined as the amount of enzyme required to produce 1 µmole of p-nitrophenol (pNP) in 1 minute at 25°C pH 6.5 from p-nitrophenyl-alpha-D-galactopyranoside.
Purity
α(1-3,6) galactosidase is tested for contaminating protease as follows; 10 μg of denatured BSA is incubated for 24 hours at 37°C with 2 μL of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation.
The production host strain has been extensively tested and does not produce any detectable glycosidases.of the BSA band after SDS-PAGE should show no evidence of degradation.
Directions for use
1. Add up to 100 µg of asialoglycoprotein or 1 nmol of oligosaccharide to tube.
2. Add water to 13 µl and 4 µl 5X Reaction Buffer.
3. Add 2 µl alpha-(1-3,6)-Galactosidase.
4. Incubate at 37°C for 1 hour. Longer incubations are necessary if fucose is present on the penultimate sugar.
Applications
Structural analysis of oligosaccharides
Xenograft transplantation studies
Removing heterogeneity from glycoproteins
规格
产品名称
Alpha Galactosidase
别名
α-半乳糖苷酶
英文别名
α-D-galactoside galactohydrolase, melibiase
规格或纯度
Specific Activity ＞30 U/mg;Activity 80 U/ml
生物活性
80 U/ml
酶学委员会编号
3.2.1.22
分子类型
酶
产品线
biological_products
储存与运输
浓度
Specific Activity ＞30 U/mg;Activity 80 U/ml
储存条件
2-8°C储存
运输条件
低温运输
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
常见问题
本产品应如何储存？
请于2–8 °C条件下保存。需冷藏保存以维持规定的有效期。
本产品如何运输？
本产品采用湿冰冷藏运输。收货后请立即拆包，并按上述储存条件保存。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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