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Alpha Mannosidase
有货
概述
Product Description
alpha-D-mannosidase, p-nitrophenyl-alpha-mannosidase, alpha-D-mannopyranosidase, alpha mannosidase, exo-alpha-mannosidase
Alpha Mannosidase from Jack Bean cleaves α(1-2,3,6)-linked mannose.
This enzyme is often used in conjunction with Core Alpha-(1-6) Mannosidase (E-AM02) if a noncleavable core α(1-6) mannose is present on the substrate.
Specificty
All Alpha-(1-2,3,6)-linked mannose
Purity
Each lot of α(1-2,3,6) mannosidase is tested for contaminating substances by incubating the enzyme for 24 hours at 37°C with substrates indicated in the table below. No detectable activity is evident for any of these potential contaminants. The detection limit of this assay is 5 μU/mL (IUB).
For the protease assay, 10 μg of denatured BSA is incubated for 24 hours with 2 μl of enzyme. Analysis of the BSA band after SDS-PAGE should show no evidence of degradation.
Molecular weight
two polypeptides of 64,000 and 44,000 daltons
Specific Activity Assay
One unit of Alpha Mannosidase is defined as the amount of enzyme required to hydrolyze 1 µmole of p-nitrophenyl-alpha-p-mannoside to p-nitrophenol in 1 minute at pH 5.0 and 37˚C.
Contents
Alpha-(1-2,3,6) Mannosidase in 150 mM sodium phosphate, 0.1 mM ZnCl2 pH 7.5. (pH 7.5).
Included with 20 µL and 60 µL pack sizes:
200 µl 5x Reaction Buffer 5.0 (250 mM sodium phosphate, pH 5.0).
Suggested usage
1. Add up to 1 nmol of oligosaccharide.
2. Add deionized water to 15 µl.
3. Add 4 µl 5x Reaction Buffer 5.0.
4. Add 1 µL of enzyme
5. Incubate ten minutes at 37˚C.
规格
产品名称
Alpha Mannosidase
别名
α-甘露糖苷酶
规格或纯度
Specific Activity ＞3 U/mg;Activity ＞10 U/ml
生物活性
>10 U/ml
酶学委员会编号
3.2.1.24
分子类型
小分子
产品线
生物产品
储存与运输
浓度
Specific Activity ＞3 U/mg;Activity ＞10 U/ml
储存条件
2-8°C储存
运输条件
低温运输
高级数据
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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溶液计算器
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